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dc.contributor.author
Azim, Kasum
dc.contributor.author
Fiorelli, Roberto
dc.contributor.author
Zweifel, Stefan
dc.contributor.author
Hurtado-Chong, Anahi
dc.contributor.author
Yoshikawa, Kazuaki
dc.contributor.author
Slomianka, Lutz
dc.contributor.author
Raineteau, Olivier
dc.date.accessioned
2018-10-04T09:04:46Z
dc.date.available
2017-06-10T13:54:22Z
dc.date.available
2018-10-04T09:04:46Z
dc.date.issued
2012-11-15
dc.identifier.issn
1932-6203
dc.identifier.other
10.1371/journal.pone.0049087
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/63534
dc.identifier.doi
10.3929/ethz-b-000063534
dc.description.abstract
Recent studies suggest that the subventricular zone (SVZ) of the lateral ventricle is populated by heterogeneous populations of stem and progenitor cells that, depending on their exact location, are biased to acquire specific neuronal fates. This newly described heterogeneity of SVZ stem and progenitor cells underlines the necessity to develop methods for the accurate quantification of SVZ stem and progenitor subpopulations. In this study, we provide 3-dimensional topographical maps of slow cycling “stem” cells and progenitors based on their unique cell cycle properties. These maps revealed that both cell populations are present throughout the lateral ventricle wall as well as in discrete regions of the dorsal wall. Immunodetection of transcription factors expressed in defined progenitor populations further reveals that divergent lineages have clear regional enrichments in the rostro-caudal as well as in the dorso-ventral span of the lateral ventricle. Thus, progenitors expressing Tbr2 and Dlx2 were confined to dorsal and dorso-lateral regions of the lateral ventricle, respectively, while Mash1+ progenitors were more homogeneously distributed. All cell populations were enriched in the rostral-most region of the lateral ventricle. This diversity and uneven distribution greatly impede the accurate quantification of SVZ progenitor populations. This is illustrated by measuring the coefficient of error of estimates obtained by using increasing section sampling interval. Based on our empirical data, we provide such estimates for all progenitor populations investigated in this study. These can be used in future studies as guidelines to judge if the precision obtained with a sampling scheme is sufficient to detect statistically significant differences between experimental groups if a biological effect is present. Altogether, our study underlines the need to consider the SVZ of the lateral ventricle as a complex 3D structure and define methods to accurately assess neural stem cells or progenitor diversity and population sizes in physiological or experimental paradigms.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Public Library of Science
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/3.0/
dc.title
3-Dimensional Examination of the Adult Mouse Subventricular Zone Reveals Lineage-Specific Microdomains
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 3.0 Unported
ethz.journal.title
PLoS ONE
ethz.journal.volume
7
en_US
ethz.journal.issue
11
en_US
ethz.journal.abbreviated
PLoS ONE
ethz.pages.start
e49087
en_US
ethz.size
16 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.nebis
006206116
ethz.publication.place
Lawrence, KS, USA
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich, direkt::00012 - Lehre und Forschung, direkt::00007 - Departemente, direkt::02070 - Departement Gesundheitswissenschaften und Technologie / Department of Health Sciences and Technology::03481 - Schwab, Martin (emeritus)::08683 - Gruppe Raineteau
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich, direkt::00012 - Lehre und Forschung, direkt::00007 - Departemente, direkt::02070 - Departement Gesundheitswissenschaften und Technologie / Department of Health Sciences and Technology::03481 - Schwab, Martin (emeritus)::08683 - Gruppe Raineteau
ethz.date.deposited
2017-06-10T13:55:47Z
ethz.source
ECIT
ethz.identifier.importid
imp59365056a1b6322572
ethz.ecitpid
pub:100575
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-15T00:36:55Z
ethz.rosetta.lastUpdated
2018-10-04T09:04:57Z
ethz.rosetta.versionExported
true
ethz.COinS
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