Abstract
Recent progress in protein engineering has established optogenetics as one of the leading external non-invasive stimulation strategies, with many optogenetic tools being designed for in vivo operation. Characterization and optimization of these tools require a high-throughput and versatile light delivery system targeting micro-titer culture volumes. Here, we present a universal light illumination platform – Diya, compatible with a wide range of cell culture plates and dishes. Diya hosts specially designed features ensuring active thermal management, homogeneous illumination, and minimal light bleedthrough. It offers light induction programming via a user-friendly custom-designed GUI. Through extensive characterization experiments with multiple optogenetic tools in diverse model organisms (bacteria, yeast, and human cell lines), we show that Diya maintains viable conditions for cell cultures undergoing light induction. Finally, we demonstrate an optogenetic strategy for in vivo biomolecular controller operation. With a custom-designed antithetic integral feedback circuit, we exhibit robust perfect adaptation and light-controlled set-point variation using Diya. Show more
Permanent link
https://doi.org/10.3929/ethz-b-000636376Publication status
publishedExternal links
Journal / series
iScienceVolume
Pages / Article No.
Publisher
ElsevierOrganisational unit
03921 - Khammash, Mustafa / Khammash, Mustafa
Funding
743269 - Theory and Design tools for bio-molecular control systems (EC)
801041 - Cypergenetic Tissue Engineering (EC)
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