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dc.contributor.author
Le Meur, Sylvaine
dc.contributor.author
Zinn, Manfred
dc.contributor.author
Egli, Thomas
dc.contributor.author
Thöny-Meyer, Linda
dc.contributor.author
Ren, Qun
dc.date.accessioned
2019-05-31T13:04:04Z
dc.date.available
2017-06-10T14:06:09Z
dc.date.available
2019-05-31T13:04:04Z
dc.date.issued
2012-08-22
dc.identifier.issn
1472-6750
dc.identifier.other
10.1186/1472-6750-12-53
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/63814
dc.identifier.doi
10.3929/ethz-b-000063814
dc.description.abstract
Background Pseudomonas putida KT2440 is able to synthesize large amounts of medium-chain-length polyhydroxyalkanoates (mcl-PHAs). To reduce the substrate cost, which represents nearly 50% of the total PHA production cost, xylose, a hemicellulose derivate, was tested as the growth carbon source in an engineered P. putida KT2440 strain. Results The genes encoding xylose isomerase (XylA) and xylulokinase (XylB) from Escherichia coli W3110 were introduced into P. putida KT2440. The recombinant KT2440 exhibited a XylA activity of 1.47 U and a XylB activity of 0.97 U when grown on a defined medium supplemented with xylose. The cells reached a maximum specific growth rate of 0.24 h-1 and a final cell dry weight (CDW) of 2.5 g L-1 with a maximal yield of 0.5 g CDW g-1 xylose. Since no mcl-PHA was accumulated from xylose, mcl-PHA production can be controlled by the addition of fatty acids leading to tailor-made PHA compositions. Sequential feeding strategy was applied using xylose as the growth substrate and octanoic acid as the precursor for mcl-PHA production. In this way, up to 20% w w-1 of mcl-PHA was obtained. A yield of 0.37 g mcl-PHA per g octanoic acid was achieved under the employed conditions. Conclusions Sequential feeding of relatively cheap carbohydrates and expensive fatty acids is a practical way to achieve more cost-effective mcl-PHA production. This study is the first reported attempt to produce mcl-PHA by using xylose as the growth substrate. Further process optimizations to achieve higher cell density and higher productivity of mcl-PHA should be investigated. These scientific exercises will undoubtedly contribute to the economic feasibility of mcl-PHA production from renewable feedstock.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
London
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/2.0/
dc.subject
mcl-PHA
en_US
dc.subject
Xylose
en_US
dc.subject
Octanoic acid
en_US
dc.subject
Pseudomonas putida KT2440
en_US
dc.subject
Sequential-feeding
en_US
dc.subject
Tailor-made PHA
en_US
dc.title
Production of medium-chain-length polyhydroxyalkanoates by sequential feeding of xylose and octanoic acid in engineered Pseudomonas putida KT2440
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 2.0 Generic
ethz.journal.title
BMC Biotechnology
ethz.journal.volume
12
en_US
ethz.journal.issue
1
en_US
ethz.journal.abbreviated
BMC biotechnol. (Online)
ethz.pages.start
53
en_US
ethz.size
12 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.nebis
004406117
ethz.publication.place
BioMed Central
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2017-06-10T14:09:27Z
ethz.source
ECIT
ethz.identifier.importid
imp5936505b7ca7933499
ethz.ecitpid
pub:101118
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-15T20:50:40Z
ethz.rosetta.lastUpdated
2019-05-31T13:04:15Z
ethz.rosetta.versionExported
true
ethz.COinS
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