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dc.contributor.author
Xu, Shanshan
dc.contributor.author
Chen, Guangdi
dc.contributor.author
Chen, Chunjing
dc.contributor.author
Sun, Chuan
dc.contributor.author
Zhang, Danying
dc.contributor.author
Murbach, Manuel
dc.contributor.author
Kuster, Niels
dc.contributor.author
Zeng, Qunli
dc.contributor.author
Xu, Zhengping
dc.date.accessioned
2018-08-23T08:57:38Z
dc.date.available
2017-06-10T14:24:58Z
dc.date.available
2018-08-23T08:57:38Z
dc.date.issued
2013-01-23
dc.identifier.issn
1932-6203
dc.identifier.other
10.1371/journal.pone.0054906
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/64177
dc.identifier.doi
10.3929/ethz-b-000064177
dc.description.abstract
Background Although IARC clarifies radiofrequency electromagnetic fields (RF-EMF) as possible human carcinogen, the debate on its health impact continues due to the inconsistent results. Genotoxic effect has been considered as a golden standard to determine if an environmental factor is a carcinogen, but the currently available data for RF-EMF remain controversial. As an environmental stimulus, the effect of RF-EMF on cellular DNA may be subtle. Therefore, more sensitive method and systematic research strategy are warranted to evaluate its genotoxicity. Objectives To determine whether RF-EMF does induce DNA damage and if the effect is cell-type dependent by adopting a more sensitive method γH2AX foci formation; and to investigate the biological consequences if RF-EMF does increase γH2AX foci formation. Methods Six different types of cells were intermittently exposed to GSM 1800 MHz RF-EMF at a specific absorption rate of 3.0 W/kg for 1 h or 24 h, then subjected to immunostaining with anti-γH2AX antibody. The biological consequences in γH2AX-elevated cell type were further explored with comet and TUNEL assays, flow cytometry, and cell growth assay. Results Exposure to RF-EMF for 24 h significantly induced γH2AX foci formation in Chinese hamster lung cells and Human skin fibroblasts (HSFs), but not the other cells. However, RF-EMF-elevated γH2AX foci formation in HSF cells did not result in detectable DNA fragmentation, sustainable cell cycle arrest, cell proliferation or viability change. RF-EMF exposure slightly but not significantly increased the cellular ROS level. Conclusions RF-EMF induces DNA damage in a cell type-dependent manner, but the elevated γH2AX foci formation in HSF cells does not result in significant cellular dysfunctions.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Public Library of Science
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/3.0/
dc.title
Cell Type-Dependent Induction of DNA Damage by 1800 MHz Radiofrequency Electromagnetic Fields Does Not Result in Significant Cellular Dysfunctions
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 3.0 Unported
ethz.journal.title
PLoS ONE
ethz.journal.volume
8
en_US
ethz.journal.issue
1
en_US
ethz.journal.abbreviated
PLoS ONE
ethz.pages.start
e54906
en_US
ethz.size
10 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.nebis
006206116
ethz.publication.place
Lawrence, KS, USA
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2017-06-10T14:25:37Z
ethz.source
ECIT
ethz.identifier.importid
imp59365063a084361186
ethz.ecitpid
pub:101920
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-14T18:30:23Z
ethz.rosetta.lastUpdated
2018-08-23T08:57:41Z
ethz.rosetta.versionExported
true
ethz.COinS
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