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dc.contributor.author
Islam, Shofiqul Md.
dc.contributor.author
Studer, Bruno
dc.contributor.author
Byrne, Stephen L.
dc.contributor.author
Farrell, Jacqueline D.
dc.contributor.author
Panitz, Frank
dc.contributor.author
Bendixen, Christian
dc.contributor.author
Møller, Ian M.
dc.contributor.author
Asp, Torben
dc.date.accessioned
2018-10-09T11:50:36Z
dc.date.available
2017-06-10T14:33:54Z
dc.date.available
2018-10-09T11:50:36Z
dc.date.issued
2013-03
dc.identifier.other
10.1186/1471-2164-14-202
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/64341
dc.identifier.doi
10.3929/ethz-b-000064341
dc.description.abstract
Background Perennial ryegrass (Lolium perenne L.) is one of the most important forage and turf grass species of temperate regions worldwide. Its mitochondrial genome is inherited maternally and contains genes that can influence traits of agricultural importance. Moreover, the DNA sequence of mitochondrial genomes has been established and compared for a large number of species in order to characterize evolutionary relationships. Therefore, it is crucial to understand the organization of the mitochondrial genome and how it varies between and within species. Here, we report the first de novo assembly and annotation of the complete mitochondrial genome from perennial ryegrass. Results Intact mitochondria from perennial ryegrass leaves were isolated and used for mtDNA extraction. The mitochondrial genome was sequenced to a 167-fold coverage using the Roche 454 GS-FLX Titanium platform, and assembled into a circular master molecule of 678,580 bp. A total of 34 proteins, 14 tRNAs and 3 rRNAs are encoded by the mitochondrial genome, giving a total gene space of 48,723 bp (7.2%). Moreover, we identified 149 open reading frames larger than 300 bp and covering 67,410 bp (9.93%), 250 SSRs, 29 tandem repeats, 5 pairs of large repeats, and 96 pairs of short inverted repeats. The genes encoding subunits of the respiratory complexes – nad1 to nad9, cob, cox1 to cox3 and atp1 to atp9 – all showed high expression levels both in absolute numbers and after normalization. Conclusions The circular master molecule of the mitochondrial genome from perennial ryegrass presented here constitutes an important tool for future attempts to compare mitochondrial genomes within and between grass species. Our results also demonstrate that mitochondria of perennial ryegrass contain genes crucial for energy production that are well conserved in the mitochondrial genome of monocotyledonous species. The expression analysis gave us first insights into the transcriptome of these mitochondrial genes in perennial ryegrass.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
BioMed Central
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/2.0/
dc.subject
De novo assembly
en_US
dc.subject
Mitochondrial gene expression
en_US
dc.subject
Mitochondrial genome
en_US
dc.subject
Next-generation sequencing
en_US
dc.subject
Perennial ryegrass (Lolium perenne L.)
en_US
dc.title
The genome and transcriptome of perennial ryegrass mitochondria
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 2.0 Generic
ethz.journal.title
BMC Genomics
ethz.journal.volume
14
en_US
ethz.pages.start
202
en_US
ethz.size
21 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.nebis
004256340
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02703 - Institut für Agrarwissenschaften / Institute of Agricultural Sciences::03969 - Studer, Bruno / Studer, Bruno
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02703 - Institut für Agrarwissenschaften / Institute of Agricultural Sciences::03969 - Studer, Bruno / Studer, Bruno
ethz.date.deposited
2017-06-10T14:35:29Z
ethz.source
ECIT
ethz.identifier.importid
imp59365066f200572000
ethz.ecitpid
pub:102203
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-13T19:26:45Z
ethz.rosetta.lastUpdated
2020-02-15T15:19:52Z
ethz.rosetta.versionExported
true
ethz.COinS
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