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dc.contributor.author
Ruts, Tom
dc.contributor.author
Matsubara, Shizue
dc.contributor.author
Walter, Achim
dc.date.accessioned
2019-04-25T11:40:06Z
dc.date.available
2017-06-10T14:44:09Z
dc.date.available
2019-04-25T11:40:06Z
dc.date.issued
2013
dc.identifier.issn
1746-4811
dc.identifier.other
10.1186/1746-4811-9-2
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/64552
dc.identifier.doi
10.3929/ethz-b-000064552
dc.description.abstract
Background Root growth is highly responsive to temporal changes in the environment. On the contrary, diel (24 h) leaf expansion in dicot plants is governed by endogenous control and therefore its temporal pattern does not strictly follow diel changes in the environment. Nevertheless, root and shoot are connected with each other through resource partitioning and changing environments for one organ could affect growth of the other organ, and hence overall plant growth. Results We developed a new technique, GROWMAP-plant, to monitor growth processes synchronously in leaf and root of the same plant with a high resolution over the diel period. This allowed us to quantify treatment effects on the growth rates of the treated and non-treated organ and the possible interaction between them. We subjected the root system of Nicotiana tabacum seedlings to three different conditions: constant darkness at 22°C (control), constant darkness at 10°C (root cooling), and 12 h/12 h light–dark cycles at 22°C (root illumination). In all treatments the shoot was kept under the same 12 h/12 h light–dark cycles at 22°C. Root growth rates were found to be constant when the root-zone environment was kept constant, although the root cooling treatment significantly reduced root growth. Root velocity was decreased after light-on and light-off events of the root illumination treatment, resulting in diel root growth rhythmicity. Despite these changes in root growth, leaf growth was not affected substantially by the root-zone treatments, persistently showing up to three times higher nocturnal growth than diurnal growth. Conclusion GROWMAP-plant allows detailed synchronous growth phenotyping of leaf and root in the same plant. Root growth was very responsive to the root cooling and root illumination, while these treatments altered neither relative growth rate nor diel growth pattern in the seedling leaf. Our results that were obtained simultaneously in growing leaves and roots of the same plants corroborate the high sensitivity of root growth to the environment and the contrasting robustness of diel growth patterns in dicot leaves. Further, they also underpin the importance to carefully control the experimental conditions for root growth analysis to avoid or/and minimize artificial complications.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
BioMed Central
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/2.0/
dc.subject
Growth
en_US
dc.subject
Phenotyping
en_US
dc.subject
Diel
en_US
dc.subject
Diurnal
en_US
dc.subject
Temperature
en_US
dc.subject
Leaf
en_US
dc.subject
Shoot
en_US
dc.subject
Root
en_US
dc.subject
Nicotiana tabacum
en_US
dc.subject
Circadian
en_US
dc.title
Synchronous high-resolution phenotyping of leaf and root growth in Nicotiana tabacum over 24-h periods with GROWMAP-plant
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 2.0 Generic
dc.date.published
2013-01-23
ethz.journal.title
Plant Methods
ethz.journal.volume
9
en_US
ethz.journal.abbreviated
Plant methods
ethz.pages.start
2
en_US
ethz.size
8 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02703 - Institut für Agrarwissenschaften / Institute of Agricultural Sciences::03894 - Walter, Achim / Walter, Achim
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02703 - Institut für Agrarwissenschaften / Institute of Agricultural Sciences::03894 - Walter, Achim / Walter, Achim
ethz.date.deposited
2017-06-10T14:45:47Z
ethz.source
ECIT
ethz.identifier.importid
imp5936506ad6bf148133
ethz.ecitpid
pub:102654
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-14T23:00:06Z
ethz.rosetta.lastUpdated
2024-02-02T07:44:07Z
ethz.rosetta.versionExported
true
ethz.COinS
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