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dc.contributor.author
Goettelmann, Florian
dc.contributor.author
Koebnik, Ralf
dc.contributor.author
Roman-Reyna, Veronica
dc.contributor.author
Studer, Bruno
dc.contributor.author
Kölliker, Roland
dc.date.accessioned
2023-12-07T13:35:35Z
dc.date.available
2023-12-07T13:03:42Z
dc.date.available
2023-12-07T13:35:35Z
dc.date.issued
2023
dc.identifier.issn
1471-2164
dc.identifier.other
10.1186/s12864-023-09855-8
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/646101
dc.identifier.doi
10.3929/ethz-b-000646101
dc.description.abstract
Background Xanthomonas translucens pv. graminis (Xtg) is a major bacterial pathogen of economically important forage grasses, causing severe yield losses. So far, genomic resources for this pathovar consisted mostly of draft genome sequences, and only one complete genome sequence was available, preventing comprehensive comparative genomic analyses. Such comparative analyses are essential in understanding the mechanisms involved in the virulence of pathogens and to identify virulence factors involved in pathogenicity. Results In this study, we produced high-quality, complete genome sequences of four strains of Xtg, complementing the recently obtained complete genome sequence of the Xtg pathotype strain. These genomic resources allowed for a comprehensive comparative analysis, which revealed a high genomic plasticity with many chromosomal rearrangements, although the strains were highly related. A high number of transposases were exclusively found in Xtg and corresponded to 413 to 457 insertion/excision transposable elements per strain. These mobile genetic elements are likely to be involved in the observed genomic plasticity and may play an important role in the adaptation of Xtg. The pathovar was found to lack a type IV secretion system, and it possessed the smallest set of type III effectors in the species. However, three XopE and XopX family effectors were found, while in the other pathovars of the species two or less were present. Additional genes that were specific to the pathovar were identified, including a unique set of minor pilins of the type IV pilus, 17 TonB-dependent receptors (TBDRs), and 11 plant cell wall degradative enzymes. Conclusion These results suggest a high adaptability of Xtg, conferred by the abundance of mobile genetic elements, which could play a crucial role in pathogen adaptation. The large amount of such elements in Xtg compared to other pathovars of the species could, at least partially, explain its high virulence and broad host range. Conserved features that were specific to Xtg were identified, and further investigation will help to determine genes that are essential to pathogenicity and host adaptation of Xtg.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
BioMed Central
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Xanthomonas translucens pv. graminis
en_US
dc.subject
Complete genomes
en_US
dc.subject
Phylogeny
en_US
dc.subject
Host adaptation
en_US
dc.subject
Comparative genomics
en_US
dc.subject
Virulence factors
en_US
dc.title
High genomic plasticity and unique features of Xanthomonas translucens pv. graminis revealed through comparative analysis of complete genome sequences
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2023-12-05
ethz.journal.title
BMC Genomics
ethz.journal.volume
24
en_US
ethz.journal.issue
1
en_US
ethz.journal.abbreviated
BMC Genomics
ethz.pages.start
741
en_US
ethz.size
14 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.grant
Resistance gene identification through advanced host - pathogen genomics in the Xanthomonas - ryegrass pathosystem
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02703 - Institut für Agrarwissenschaften / Institute of Agricultural Sciences::03969 - Studer, Bruno / Studer, Bruno
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02703 - Institut für Agrarwissenschaften / Institute of Agricultural Sciences::03969 - Studer, Bruno / Studer, Bruno
en_US
ethz.grant.agreementno
177062
ethz.grant.fundername
SNF
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.program
COST
ethz.date.deposited
2023-12-07T13:03:42Z
ethz.source
FORM
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2023-12-07T13:35:36Z
ethz.rosetta.lastUpdated
2024-02-03T07:54:56Z
ethz.rosetta.versionExported
true
ethz.COinS
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