Extended-spectrum β-lactamase-producing Enterobacterales in diverse foodstuffs: a prospective, longitudinal study in the city of Basel, Switzerland
dc.contributor.author
Gomez Sanz, Elena
dc.contributor.author
Bagutti, Claudia
dc.contributor.author
García-Martín, Ana B.
dc.contributor.author
Roth, Jan A.
dc.contributor.author
Alt Hug, Monica
dc.contributor.author
Maurer Pekerman, Laura
dc.contributor.author
Schindler, Ruth
dc.contributor.author
Furger, Reto
dc.contributor.author
Eichenberger, Lucas
dc.contributor.author
Steffen, Ingrid
dc.contributor.author
Hübner, Philipp
dc.contributor.author
Stadler, Tanja
dc.contributor.author
Aguilar-Bultet, Lisandra
dc.contributor.author
Tschudin-Sutter, Sarah
dc.date.accessioned
2023-12-18T17:13:02Z
dc.date.available
2023-12-15T09:02:11Z
dc.date.available
2023-12-18T17:13:02Z
dc.date.issued
2023
dc.identifier.issn
1664-302X
dc.identifier.other
10.3389/fmicb.2023.1295037
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/647821
dc.identifier.doi
10.3929/ethz-b-000647821
dc.description.abstract
Background: The involvement of non-human-to-human transmission of extended-spectrum beta-lactamase-producing Enterobacterales (ESBL-PE) remains elusive. Foodstuffs may serve as reservoirs for ESBL-PE and contribute to their spread.Aim: We aimed to systematically investigate the presence and spatiotemporal distribution of ESBL-PE in diverse unprocessed foodstuffs of different origin purchased in a central European city.Methods: Chicken and green (herbs, salad, sprouts, vegetables) samples were collected monthly for two consecutive years, from June 2017 to June 2019, from large supermarket chains and small local food retailers, representing all ten postcode areas of the City of Basel (Switzerland), and the kitchen of the University Hospital Basel (Basel, Switzerland). After enrichment, presumptive ESBL-PE were isolated by selective culture methods and identified by Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. ESBL production was confirmed by phenotypic testing.Results: Among 947 food samples, 14.8% were positive for ESBL-PE isolate/s belonging to eight different ESBL-producing bacterial species. Escherichia coli and Serratia fonticola were predominant across samples (9 and 2%, respectively). Higher ESBL-PE prevalence was observed in chicken (25.9%) than in green (3.8%) samples (p < 0.001). Among greens, ESBL-PE were most frequently isolated from sprouts (15.2%). High ESBL-PE species diversity was observed among chicken samples, with E. coli as predominant (17.6%). ESBL-producing Enterobacter cloacae was detected among different greens. Yet, ESBL-producing Klebsiella pneumoniae was predominant in sprouts (12.1%). In total, 20.5% of samples from organic farming and 14.2% of samples from conventionally raised animals harbored an ESBL-producing isolate. Detection of ESBL-PE across samples differed between organic and non-organic when stratified by food source (p < 0.001), particularly among greens (12.5% organic, 2.4% conventional). High proportion of organic chicken samples was positive for ESBL-E. coli (33.3%), while the detection of several species characterized the conventional chicken samples. No significant differences in ESBL-PE frequences were detected between national (13.4%) and international samples (8.0%) (p = 0.122). Instead, differences were observed between regions of food production and countries (p < 0.001). No significant differences were found when comparing the proportion of ESBL-PE positive samples across districts, shop sizes and the hospital kitchen. The percentage of ESBL-PE positive samples did not differ monthly across the two-year sampling period (p = 0.107).Conclusion: Our findings indicate moderate dissemination of ESBL-PE in foodstuffs, especially between chicken products and sprouts. Chicken meat represents a source for several ESBL-producing Enterobacterales, especially E. coli, while greens are more prone to carry ESBL-K. pneumoniae and E. cloacae. We disclose the importance of food type, food production system and production origin when assessing the risk of contamination with different ESBL-PE species.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Frontiers Media
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
ESBL-producing Enterobacterales
en_US
dc.subject
foodstuff, chicken, greens
en_US
dc.subject
Escherichia coli
en_US
dc.subject
Klebsiella pneumoniae
en_US
dc.subject
spatiotemporal distribution
en_US
dc.title
Extended-spectrum β-lactamase-producing Enterobacterales in diverse foodstuffs: a prospective, longitudinal study in the city of Basel, Switzerland
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2023-11-22
ethz.journal.title
Frontiers in Microbiology
ethz.journal.volume
14
en_US
ethz.journal.abbreviated
Front Microbiol
ethz.pages.start
1295037
en_US
ethz.size
12 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.grant
Transmission of ESBL-producing Enterobacteriaceae and their mobile genetic elements - identification of sources by whole genome sequencing
en_US
ethz.identifier.wos
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::09490 - Stadler, Tanja / Stadler, Tanja
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::09490 - Stadler, Tanja / Stadler, Tanja
ethz.grant.agreementno
167060
ethz.grant.fundername
SNF
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.program
NFP 72: Gesuch
ethz.date.deposited
2023-12-15T09:02:28Z
ethz.source
WOS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2023-12-18T17:13:04Z
ethz.rosetta.lastUpdated
2024-02-03T08:06:39Z
ethz.rosetta.versionExported
true
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