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dc.contributor.author
Sachs, Maike K.
dc.contributor.author
Makieva, Sofia
dc.contributor.author
Velasco Gil, Ana
dc.contributor.author
Xie, Min
dc.contributor.author
Ille, Fabian
dc.contributor.author
Salvadori, Vincent
dc.contributor.author
Schmidhauser, Meret
dc.contributor.author
Saenz-de-Juano, Mara D.
dc.contributor.author
Ulbrich, Susanne E.
dc.contributor.author
Leeners, Brigitte
dc.date.accessioned
2024-02-19T10:25:07Z
dc.date.available
2024-02-19T06:57:47Z
dc.date.available
2024-02-19T10:25:07Z
dc.date.issued
2024-02
dc.identifier.issn
2666-335X
dc.identifier.other
10.1016/j.xfss.2023.11.004
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/660133
dc.description.abstract
Objective: To compare the transcriptome of human cumulus cells (CCs) from oocytes with different outcomes (pregnancy yes/no, live birth [LB] yes/no), to identify noninvasive biomarkers for oocyte selection as well as new therapeutic targets to increase LB rates from assisted reproductive technologies (ART). Design: Retrospective observational study. Settings: This study was conducted at a University Hospital in Switzerland. Patients: Subfertile couples undergoing controlled ovarian superstimulation and intracytoplasmic sperm injection with subsequent unbiopsied embryo transfer below the female age of 43 years. Intervention(s): RNA sequencing of CCs from oocytes results in a pregnancy, no pregnancy, LB, or no LB. Main outcome Measures: Differential gene expression (DEG) between CCs of oocytes results in “no pregnancy” vs. “pregnancy” and “pregnancy only” vs. “live birth.” Results: Although RNA sequencing did not reveal DEGs when comparing the transcriptomic profiles of the groups “no pregnancy” with “pregnancy,” we identified 139 DEGs by comparing “pregnancy only” with “live birth,” of which 28 belonged to clusters relevant to successful ART outcomes (i.e., CTGF, SERPINE2, PCK1, HHIP, HS3ST, and BIRC5). A functional enrichment analysis revealed that the transcriptome of CCs associated with LB depicts pathways of extracellular matrix, inflammatory cascades leading to ovulation, cell patterning, proliferation, and differentiation, and silencing pathways leading to apoptosis. Conclusion: We identified a CCs transcriptomic profile associated with LB after embryo transfer that, after further validation, could serve to predict successful ART outcomes. The definition of relevant pathways of CCs related to oocyte competency contributes to a broader understanding of the cumulus oocyte complex and helps identify further therapeutic targets for improving ART success.
en_US
dc.language.iso
en
en_US
dc.publisher
Elsevier
en_US
dc.title
Transcriptomic signature of luteinized cumulus cells of oocytes developing to live birth after women received intracytoplasmic sperm injection
en_US
dc.type
Journal Article
dc.date.published
2023-11-23
ethz.journal.title
F&S Science
ethz.journal.volume
5
en_US
ethz.journal.issue
1
en_US
ethz.pages.start
24
en_US
ethz.pages.end
38
en_US
ethz.identifier.scopus
ethz.publication.status
published
en_US
ethz.date.deposited
2024-02-19T06:57:48Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Metadata only
en_US
ethz.rosetta.installDate
2024-02-19T10:25:08Z
ethz.rosetta.lastUpdated
2024-02-19T10:25:08Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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