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dc.contributor.author
Palo, Benedetta Di
dc.contributor.author
Rippa, Valentina
dc.contributor.author
Santi, Isabella
dc.contributor.author
Brettoni, Cecilia
dc.contributor.author
Muzzi, Alessandro
dc.contributor.author
Metruccio, Matteo Maria Emiliano
dc.contributor.author
Grifantini, Renata
dc.contributor.author
Telford, John L.
dc.contributor.author
Paccani, Silvia R.
dc.contributor.author
Soriani, Marco
dc.date.accessioned
2018-10-04T08:14:07Z
dc.date.available
2017-06-10T16:24:30Z
dc.date.available
2018-10-04T08:14:07Z
dc.date.issued
2013-04-09
dc.identifier.issn
1932-6203
dc.identifier.other
10.1371/journal.pone.0061294
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/66188
dc.identifier.doi
10.3929/ethz-b-000066188
dc.description.abstract
Although the contribution of carbohydrate catabolism to bacterial colonization and infection is well recognized, the transcriptional changes during these processes are still unknown. In this study, we have performed comparative global gene expression analysis of GBS in sugar-free versus high glucose milieu. The analysis revealed a differential expression of genes involved in metabolism, transport and host-pathogen interaction. Many of them appeared to be among the genes previously reported to be controlled by the CovRS two-component system. Indeed, the transcription profile of a ΔcovRS strain grown in high-glucose conditions was profoundly affected. In particular, of the total genes described to be regulated by glucose, ∼27% were under CovRS control with a functional role in protein synthesis, transport, energy metabolism and regulation. Among the CovRS dependent genes, we found bibA, a recently characterized adhesin involved in bacterial serum resistance and here reported to be down-regulated by glucose. ChIP analysis revealed that in the presence of glucose, CovR binds bibA promoter in vivo, suggesting that CovR may act as a negative regulator or a repressor. We also demonstrated that, as for other target promoters, chemical phosphorylation of CovR in aspartic acid increases its affinity for the bibA promoter region. The data reported in this study contribute to the understanding of the molecular mechanisms modulating the adaptation of GBS to glucose.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Public Library of Science
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/3.0/
dc.title
Adaptive Response of Group B Streptococcus to High Glucose Conditions: New Insights on the CovRS Regulation Network
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 3.0 Unported
ethz.journal.title
PLoS ONE
ethz.journal.volume
8
en_US
ethz.journal.issue
4
en_US
ethz.journal.abbreviated
PLoS ONE
ethz.pages.start
e61294
en_US
ethz.size
11 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.nebis
006206116
ethz.publication.place
Lawrence, KS, USA
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2017-06-10T16:26:10Z
ethz.source
ECIT
ethz.identifier.importid
imp5936508b9a6fc98209
ethz.ecitpid
pub:105526
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-20T18:09:22Z
ethz.rosetta.lastUpdated
2018-10-04T08:14:17Z
ethz.rosetta.versionExported
true
ethz.COinS
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