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dc.contributor.author
Cleusix, Valentine
dc.contributor.author
Lacroix, Christophe
dc.contributor.author
Vollenweider, Sabine
dc.contributor.author
Duboux, Marc
dc.contributor.author
Le Blay, Gwenaëlle
dc.date.accessioned
2019-04-26T12:41:53Z
dc.date.available
2017-06-08T17:28:26Z
dc.date.available
2019-04-26T12:41:53Z
dc.date.issued
2007
dc.identifier.issn
1471-2180
dc.identifier.other
10.1186/1471-2180-7-101
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/6629
dc.identifier.doi
10.3929/ethz-b-000006629
dc.description.abstract
Background Reuterin produced from glycerol by Lactobacillus reuteri, a normal inhabitant of the human intestine, is a broad-spectrum antimicrobial agent. It has been postulated that reuterin could play a role in the probiotic effects of Lb. reuteri. Reuterin is active toward enteropathogens, yeasts, fungi, protozoa and viruses, but its effect on commensal intestinal bacteria is unknown. Moreover reuterin's mode of action has not yet been elucidated. Glutathione, a powerful antioxidant, which also plays a key role in detoxifying reactive aldehydes, protects certain bacteria from oxidative stress, and could also be implicated in resistance to reuterin. The aim of this work was to test the activity of reuterin against a representative panel of intestinal bacteria and to study a possible correlation between intracellular low molecular weight thiols (LMW-SH) such as glutathione, hydrogen peroxide and/or reuterin sensitivity. Reuterin was produced by Lb. reuteri SD2112 in pure glycerol solution, purified and used to test the minimal inhibitory (MIC) and minimal bactericidal concentrations (MBC). Hydrogen peroxide sensitivity and intracellular LMW-SH concentration were also analysed. Results Our data showed that most tested intestinal bacteria showed MIC below that for a sensitive indicator Escherichia coli (7.5–15 mM). Lactobacilli and Clostridium clostridioforme were more resistant with MIC ranging from 15 to 50 mM. No correlation between bacterial intracellular concentrations of LMW-SH, including glutathione, and reuterin or hydrogen peroxide sensitivities were found. Conclusion Our data showed that intestinal bacteria were very sensitive to reuterin and that their intracellular concentration of LMW-SH was not directly linked to their capacity to resist reuterin or hydrogen peroxide. This suggests that detoxification by LMW-SH such as glutathione is not a general mechanism and that other mechanisms are probably involved in bacterial tolerance to reuterin and hydrogene peroxide.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
BioMed Central
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/2.0/
dc.subject
Lactobacillus
en_US
dc.subject
Lactic Acid Bacterium
en_US
dc.subject
Intestinal Bacterium
en_US
dc.subject
Minimal Bactericidal Concentration
en_US
dc.subject
Bifidobacterium Longum
en_US
dc.title
Inhibitory activity spectrum of reuterin produced by Lactobacillus reuteri against intestinal bacteria
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 2.0 Generic
dc.date.published
2007-11-12
ethz.journal.title
BMC Microbiology
ethz.journal.volume
7
en_US
ethz.journal.abbreviated
BMC microbiol. (Online)
ethz.pages.start
101
en_US
ethz.size
9 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.nebis
004100852
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02701 - Inst.f. Lebensmittelwiss.,Ernährung,Ges. / Institute of Food, Nutrition, and Health::03626 - Lacroix, Christophe / Lacroix, Christophe
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02701 - Inst.f. Lebensmittelwiss.,Ernährung,Ges. / Institute of Food, Nutrition, and Health::03626 - Lacroix, Christophe / Lacroix, Christophe
ethz.date.deposited
2017-06-08T17:28:40Z
ethz.source
ECIT
ethz.identifier.importid
imp59364ba509e1e50596
ethz.ecitpid
pub:17070
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-26T19:00:48Z
ethz.rosetta.lastUpdated
2019-04-26T12:41:57Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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