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dc.contributor.author
Gkountidi, Anastasia-Olga
dc.contributor.author
Jahromi, Neda Haghayegh
dc.contributor.author
Gkountidi, Anastasia-Olga
dc.contributor.author
Collado-Diaz, Victor
dc.contributor.author
Blatter, Katharina
dc.contributor.author
Bauer, Aline
dc.contributor.author
Zambounis, Lito
dc.contributor.author
Medina Sanchez, Jessica Danielly
dc.contributor.author
Russo, Erica
dc.contributor.author
Runge, Peter
dc.contributor.author
Restivo, Gaetana
dc.contributor.author
Gousopoulos, Epameinondas
dc.contributor.author
Lindenblatt, Nicole
dc.contributor.author
Levesque, Mitchell P.
dc.contributor.author
Halin, Cornelia
dc.date.accessioned
2024-03-22T12:13:16Z
dc.date.available
2024-03-22T10:18:19Z
dc.date.available
2024-03-22T11:43:42Z
dc.date.available
2024-03-22T12:13:16Z
dc.date.issued
2024-03-22
dc.identifier.uri
http://hdl.handle.net/20.500.11850/665599
dc.identifier.doi
10.3929/ethz-b-000665599
dc.description.abstract
Dendritic cell (DC) migration from peripheral tissues via afferent lymphatic vessels to draining lymph nodes (dLNs) is important for the organism’s immune regulation and immune protection. Several lymphatic endothelial cell (LEC)-expressed adhesion molecules have thus far been found to support transmigration and movement within the lymphatic vasculature. In this study, we investigated the contribution of CD112, an adhesion molecule that we recently found to be highly expressed in murine LECs, to this process. Performing in vitro assays in the murine system, we found that transmigration of bone marrow-derived dendritic cells (BM-DCs) across or adhesion to murine LEC monolayers was reduced when CD112 was absent on LECs, DCs, or both cell types, suggesting the involvement of homophilic CD112–CD112 interactions. While CD112 was highly expressed in murine dermal LECs, CD112 levels were low in endogenous murine dermal DCs and BM-DCs. This might explain why we observed no defect in the in vivo lymphatic migration of adoptively transferred BM-DCs or endogenous DCs from the skin to dLNs. Compared to murine DCs, human monocyte-derived DCs expressed higher CD112 levels, and their migration across human CD112-expressing LECs was significantly reduced upon CD112 blockade. CD112 expression was also readily detected in endogenous human dermal DCs and LECs by flow cytometry and immunofluorescence. Upon incubating human skin punch biopsies in the presence of CD112-blocking antibodies, DC emigration from the tissue into the culture medium was significantly reduced, indicating impaired lymphatic migration. Overall, our data reveal a contribution of CD112 to human DC migration.
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application/zip
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application/octet-stream
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text/csv
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dc.format
image/jpeg
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dc.format
application/json
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dc.format
application/xml
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dc.format
image/tiff
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dc.format
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet
en_US
dc.language.iso
en
en_US
dc.publisher
ETH Zurich
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc-sa/4.0/
dc.subject
CD112
en_US
dc.subject
Nectin-2
en_US
dc.subject
Dendritic cells
en_US
dc.subject
Lymphatic endothelial cells
en_US
dc.subject
Lymphatic migration
en_US
dc.subject
Human
en_US
dc.title
CD112 Supports Lymphatic Migration of Human Dermal Dendritic Cells
en_US
dc.type
Dataset
dc.rights.license
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International
ethz.book.title
CD112 Supports Lymphatic Migration of Human Dermal Dendritic Cells
en_US
ethz.size
24.16 GB
en_US
ethz.publication.place
Zurich
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02534 - Institut für Pharmazeutische Wiss. / Institute of Pharmaceutical Sciences::03816 - Halin Winter, Cornelia / Halin Winter, Cornelia
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ethz.date.retentionend
indefinite
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ethz.date.retentionendDate
n/a
ethz.relation.isSupplementTo
10.3929/ethz-b-000662635
ethz.date.deposited
2024-03-22T10:18:19Z
ethz.source
FORM
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2024-03-22T12:21:25Z
ethz.rosetta.lastUpdated
2024-03-22T12:21:25Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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