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dc.contributor.author
Gerosa, Luca
dc.contributor.author
Kochanowski, Karl
dc.contributor.author
Heinemann, Matthias
dc.contributor.author
Sauer, Uwe
dc.date.accessioned
2018-06-07T13:36:48Z
dc.date.available
2017-06-10T16:53:25Z
dc.date.available
2018-06-07T13:36:48Z
dc.date.issued
2013-01
dc.identifier.other
10.1038/msb.2013.14
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/66744
dc.identifier.doi
10.3929/ethz-b-000066744
dc.description.abstract
Gene expression is regulated by specific transcriptional circuits but also by the global expression machinery as a function of growth. Simultaneous specific and global regulation thus constitutes an additional—but often neglected—layer of complexity in gene expression. Here, we develop an experimental‐computational approach to dissect specific and global regulation in the bacterium Escherichia coli. By using fluorescent promoter reporters, we show that global regulation is growth rate dependent not only during steady state but also during dynamic changes in growth rate and can be quantified through two promoter‐specific parameters. By applying our approach to arginine biosynthesis, we obtain a quantitative understanding of both specific and global regulation that allows accurate prediction of the temporal response to simultaneous perturbations in arginine availability and growth rate. We thereby uncover two principles of joint regulation: (i) specific regulation by repression dominates the transcriptional response during metabolic steady states, largely repressing the biosynthesis genes even when biosynthesis is required and (ii) global regulation sets the maximum promoter activity that is exploited during the transition between steady states.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature Publ. Group
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc-sa/3.0/
dc.subject
Expression machinery
en_US
dc.subject
Modelling
en_US
dc.subject
Synthetic biology
en_US
dc.subject
Transcriptional circuit
en_US
dc.subject
Transcriptional regulation
en_US
dc.title
Dissecting specific and global transcriptional regulation of bacterial gene expression
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported
dc.date.published
2013-04-16
ethz.journal.title
Molecular Systems Biology
ethz.journal.volume
9
en_US
ethz.journal.issue
1
en_US
ethz.pages.start
658
en_US
ethz.size
11 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.nebis
004931271
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::03713 - Sauer, Uwe / Sauer, Uwe
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::03713 - Sauer, Uwe / Sauer, Uwe
ethz.date.deposited
2017-06-10T16:57:45Z
ethz.source
ECIT
ethz.identifier.importid
imp59365096c24e664422
ethz.ecitpid
pub:106382
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-20T18:49:44Z
ethz.rosetta.lastUpdated
2018-11-07T11:29:59Z
ethz.rosetta.versionExported
true
ethz.COinS
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