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dc.contributor.author
Zampar, Guillermo G.
dc.contributor.author
Kümmel, Anne
dc.contributor.author
Ewald, Jennifer
dc.contributor.author
Jol, Stefan
dc.contributor.author
Niebel, Bastian
dc.contributor.author
Picotti, Paola
dc.contributor.author
Aebersold, Ruedi
dc.contributor.author
Sauer, Uwe
dc.contributor.author
Zamboni, Nicola
dc.contributor.author
Heinemann, Matthias
dc.date.accessioned
2018-06-07T15:21:15Z
dc.date.available
2017-06-10T16:53:28Z
dc.date.available
2018-06-07T15:21:15Z
dc.date.issued
2013-01
dc.identifier.other
10.1038/msb.2013.11
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/66745
dc.identifier.doi
10.3929/ethz-b-000066745
dc.description.abstract
The diauxic shift in Saccharomyces cerevisiae is an ideal model to study how eukaryotic cells readjust their metabolism from glycolytic to gluconeogenic operation. In this work, we generated time‐resolved physiological data, quantitative metabolome (69 intracellular metabolites) and proteome (72 enzymes) profiles. We found that the diauxic shift is accomplished by three key events that are temporally organized: (i) a reduction in the glycolytic flux and the production of storage compounds before glucose depletion, mediated by downregulation of phosphofructokinase and pyruvate kinase reactions; (ii) upon glucose exhaustion, the reversion of carbon flow through glycolysis and onset of the glyoxylate cycle operation triggered by an increased expression of the enzymes that catalyze the malate synthase and cytosolic citrate synthase reactions; and (iii) in the later stages of the adaptation, the shutting down of the pentose phosphate pathway with a change in NADPH regeneration. Moreover, we identified the transcription factors associated with the observed changes in protein abundances. Taken together, our results represent an important contribution toward a systems‐level understanding of how this adaptation is realized.
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dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature Publ. Group
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc-sa/3.0/
dc.subject
Diauxic shift
en_US
dc.subject
Fluxome
en_US
dc.subject
Metabolome
en_US
dc.subject
Proteome
en_US
dc.subject
Saccharomyces cerevisiae
en_US
dc.title
Temporal system-level organization of the switch from glycolytic to gluconeogenic operation in yeast
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported
dc.date.published
2013-04-02
ethz.journal.title
Molecular Systems Biology
ethz.journal.volume
9
en_US
ethz.journal.issue
1
en_US
ethz.pages.start
651
en_US
ethz.size
13 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.nebis
004931271
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::03927 - Picotti, Paola / Picotti, Paola
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::03663 - Aebersold, Rudolf / Aebersold, Rudolf
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::03713 - Sauer, Uwe / Sauer, Uwe
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::03927 - Picotti, Paola / Picotti, Paola
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::03663 - Aebersold, Rudolf / Aebersold, Rudolf
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::03713 - Sauer, Uwe / Sauer, Uwe
ethz.date.deposited
2017-06-10T16:57:45Z
ethz.source
ECIT
ethz.identifier.importid
imp59365096c790e52123
ethz.ecitpid
pub:106383
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-13T19:48:48Z
ethz.rosetta.lastUpdated
2018-11-07T11:30:42Z
ethz.rosetta.versionExported
true
ethz.COinS
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