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dc.contributor.author
Vogel, Viola
dc.contributor.author
Smiley, Beth L.
dc.date.accessioned
2022-09-21T09:00:41Z
dc.date.available
2017-06-10T17:33:01Z
dc.date.available
2022-09-21T09:00:41Z
dc.date.issued
1995-08-22
dc.identifier.issn
0021-9606
dc.identifier.issn
1089-7690
dc.identifier.other
10.1063/1.470247
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/67450
dc.description.abstract
A new optical method intended for interfacial protein studies has surfaced from the observation of near UV second harmonic generation (SHG) from the aromatic amino acids. Here, the first hyperpolarizability, β=4.72±0.52×10−30 esu, of t‐butyloxycarbonyl‐L‐tryptophan is measured for SHG at 266 nm, and its polar orientation at the air–water interface is deduced. The indole ring nitrogen atom is found to point upwards at the interface. From a monolayer surface density of 1.74±0.15 adsorbate molecules/nm2, it is estimated that a minimum of 0.49 Trp residues/nm2 of surface area can be detected above the water background for this wavelength. SHG studies of tryptophan‐containing peptides and proteins based on these results have the potential to complement existing linear optical techniques by providing new information on interfacial molecular ordering.
en_US
dc.language.iso
en
en_US
dc.publisher
American Institute of Physics
en_US
dc.title
Near Uv Optical 2nd-Harmonic Generation Studies of Surface-Adsorbed Tryptophan Residues
en_US
dc.type
Journal Article
ethz.journal.title
The Journal of Chemical Physics
ethz.journal.volume
103
en_US
ethz.journal.issue
8
en_US
ethz.journal.abbreviated
J. Chem. Phys.
ethz.pages.start
3140
en_US
ethz.size
5 p.
en_US
ethz.publication.place
Melville, NY
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02540 - Institut für Translationale Medizin / Institute of Translational Medicine::03640 - Vogel, Viola / Vogel, Viola
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02540 - Institut für Translationale Medizin / Institute of Translational Medicine::03640 - Vogel, Viola / Vogel, Viola
ethz.date.deposited
2017-06-10T17:35:35Z
ethz.source
ECIT
ethz.identifier.importid
imp593650a586cf752490
ethz.ecitpid
pub:107368
ethz.eth
no
en_US
ethz.availability
Metadata only
en_US
ethz.rosetta.installDate
2017-07-19T11:18:09Z
ethz.rosetta.lastUpdated
2022-03-28T11:44:57Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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