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dc.contributor.author
Morena, Francesco
dc.contributor.author
Argentati, Chiara
dc.contributor.author
Caponi, Silvia
dc.contributor.author
Lüchtefeld, Ines
dc.contributor.author
Emiliani, Carla
dc.contributor.author
Vassalli, Massimo
dc.contributor.author
Martino, Sabata
dc.date.accessioned
2024-06-12T08:55:48Z
dc.date.available
2024-06-11T05:30:29Z
dc.date.available
2024-06-12T08:55:48Z
dc.date.issued
2024-06-15
dc.identifier.issn
2405-8440
dc.identifier.other
10.1016/j.heliyon.2024.e32458
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/677601
dc.identifier.doi
10.3929/ethz-b-000677601
dc.description.abstract
This study sheds light on a ground-breaking biochemical mechanotransduction pathway and reveals how Piezo1 channels orchestrate cell migration. We observed an increased cell migration rate in HEK293T (HEK) cells treated with Yoda1, a Piezo1 agonist, or in HEK cells overexpressing Piezo1 (HEK + P). Conversely, a significant reduction in cell motility was observed in HEK cells treated with GsMTx4 (a channel inhibitor) or upon silencing Piezo1 (HEK-P). Our findings establish a direct correlation between alterations in cell motility, Piezo1 expression, abnormal F-actin microfilament dynamics, and the regulation of Cofilin1, a protein involved in severing F-actin microfilaments. Here, the conversion of inactive pCofilin1 to active Cofilin1, mediated by the serine/threonine-protein phosphatase 2A catalytic subunit C (PP2AC), resulted in increased severing of F-actin microfilaments and enhanced cell migration in HEK + P cells compared to HEK controls. However, this effect was negligible in HEK-P and HEK cells transfected with hsa-miR-133b, which post-transcriptionally inhibited PP2AC mRNA expression. In summary, our study suggests that Piezo1 regulates cell migration through a biochemical mechanotransduction pathway involving PP2AC-mediated Cofilin1 dephosphorylation, leading to changes in F-actin microfilament dynamics.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Cell Press
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.title
Piezo1 – Serine/threonine-protein phosphatase 2A – Cofilin1 biochemical mechanotransduction axis controls F-actin dynamics and cell migration
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
dc.date.published
2024-06-05
ethz.journal.title
Heliyon
ethz.journal.volume
10
en_US
ethz.journal.issue
11
en_US
ethz.pages.start
e32458
en_US
ethz.size
15 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.status
published
en_US
ethz.date.deposited
2024-06-11T05:30:30Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2024-06-12T08:55:49Z
ethz.rosetta.lastUpdated
2024-06-12T08:55:49Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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