The estrogen-responsive B box protein: a novel enhancer of interleukin-1β secretion
Abstract
The estrogen-responsive B box protein (EBBP) and Pyrin belong to a family of structurally related proteins. While mutations in the pyrin gene cause an autoinflammatory disease, the biological function of EBBP is unknown. In this study, we identified the proinflammatory cytokine interleukin-1β (IL-1β) as an EBBP-binding partner. Furthermore, caspase-1 and NACHT, LRR and Pyrin domain containing protein (NALP) 1, two components of the recently identified inflammasome, a platform for the activation of caspase-1, also interact with EBBP. These proteins bind to the RFP domain of EBBP, suggesting that this domain of so far unknown function is an important protein-binding domain. EBBP was secreted in a caspase-1-dependent manner from cultured cells, and its secretion was enhanced by IL-1β. Vice versa, endogenous and overerexpressed EBBP increased IL-1β secretion. These results provide evidence for a role of EBBP in innate immunity by enhancing the alternative secretion pathway of IL-1β. Show more
Publication status
publishedExternal links
Journal / series
Cell Death & DifferentiationVolume
Pages / Article No.
Publisher
NatureSubject
TRIM; inflammation; secretion; interleukin-1b; caspase-1Organisational unit
03520 - Werner, Sabine / Werner, Sabine
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