Differenial_expression_data_of_four_pathogen_project

Abstract

Campylobacter jejuni, non-typhoidal Salmonella spp., Listeria monocytogenes and enteropathogenic/enterohemorrhagic Escherichia coli (EPEC/EHEC) are leading causes of food-borne illness worldwide. The mouse pathogen Citrobacter rodentium has been used to model EPEC and EHEC infection in vivo. Studies are addressing the critical role of the gut microbiome in host responses to these food-borne infections, however the comparability of data across laboratories is often negatively affected by variations in different research facilities. In this study, we used the standardized OligoMM12 microbiota mouse model for studying these pathogens. We provide evidence of robust colonization and significant pathological changes in OligoMM12 mice following infection with these pathogens. Moreover, we offer insights into pathogen-specific host responses and metabolite signatures, highlighting the advantages of a standardized mouse model for direct comparisons of factors influencing the pathogenesis of major food-borne pathogens. Notably, we reveal for the first time that C. jejuni stably colonizes OligoMM12 mice, triggering inflammation. Additionally, our comparative approach successfully identifies pathogen-specific responses, including the detection of genes uniquely associated with C. jejuni infection in humans. These findings underscore the potential of the OligoMM12 model as a versatile tool for advancing our understanding of food-borne pathogen interactions.