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dc.contributor.author
Kockmann, Tobias
dc.contributor.author
Gerstung, Moritz
dc.contributor.author
Schlumpf, Tommy
dc.contributor.author
Zhu, Xhinzhou
dc.contributor.author
Hess, Daniel
dc.contributor.author
Beerenwinkel, Niko
dc.contributor.author
Beisel, Christian
dc.contributor.author
Paro, Renato
dc.date.accessioned
2019-03-25T08:16:31Z
dc.date.available
2017-06-10T20:16:29Z
dc.date.available
2019-03-25T08:16:31Z
dc.date.issued
2013-02
dc.identifier.issn
1474-7596
dc.identifier.issn
1465-6906
dc.identifier.issn
1474-760X
dc.identifier.issn
1465-6914
dc.identifier.other
10.1186/gb-2013-14-2-r18
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/70692
dc.identifier.doi
10.3929/ethz-b-000070692
dc.description.abstract
Background The question of how cells re-establish gene expression states after cell division is still poorly understood. Genetic and molecular analyses have indicated that Trithorax group (TrxG) proteins are critical for the long-term maintenance of active gene expression states in many organisms. A generally accepted model suggests that TrxG proteins contribute to maintenance of transcription by protecting genes from inappropriate Polycomb group (PcG)-mediated silencing, instead of directly promoting transcription. Results and discussion Here we report a physical and functional interaction in Drosophila between two members of the TrxG, the histone methyltransferase ASH1 and the bromodomain and extraterminal family protein FSH. We investigated this interface at the genome level, uncovering a widespread co-localization of both proteins at promoters and PcG-bound intergenic elements. Our integrative analysis of chromatin maps and gene expression profiles revealed that the observed ASH1-FSH binding pattern at promoters is a hallmark of active genes. Inhibition of FSH-binding to chromatin resulted in global down-regulation of transcription. In addition, we found that genes displaying marks of robust PcG-mediated repression also have ASH1 and FSH bound to their promoters. Conclusions Our data strongly favor a global coactivator function of ASH1 and FSH during transcription, as opposed to the notion that TrxG proteins impede inappropriate PcG-mediated silencing, but are dispensable elsewhere. Instead, our results suggest that PcG repression needs to overcome the transcription-promoting function of ASH1 and FSH in order to silence genes.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
BioMed Central
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/2.0/
dc.subject
Drosophila melanogaster
en_US
dc.subject
Trithorax group (TrxG)
en_US
dc.subject
Absent
en_US
dc.subject
Small
en_US
dc.subject
Or homeotic discs 1 protein (ASH1)
en_US
dc.subject
Swiss-Prot:Q9VW15
en_US
dc.subject
Female sterile (1) homeotic protein (FSH)
en_US
dc.subject
Swiss-Prot:P13709
en_US
dc.subject
Transcriptional regulation
en_US
dc.subject
Epigenetic gene control
en_US
dc.title
The BET protein FSH functionally interacts with ASH1 to orchestrate global gene activity in Drosophila
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 2.0 Generic
dc.date.published
2013-02-25
ethz.journal.title
Genome Biology
ethz.journal.volume
14
en_US
ethz.journal.abbreviated
Genome biol.
ethz.pages.start
R18
en_US
ethz.size
24 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::03790 - Beerenwinkel, Niko / Beerenwinkel, Niko
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::03748 - Paro, Renato / Paro, Renato
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::03790 - Beerenwinkel, Niko / Beerenwinkel, Niko
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::03748 - Paro, Renato / Paro, Renato
ethz.date.deposited
2017-06-10T20:18:32Z
ethz.source
ECIT
ethz.identifier.importid
imp593650e63318144321
ethz.ecitpid
pub:111975
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-26T19:59:11Z
ethz.rosetta.lastUpdated
2019-03-25T08:16:55Z
ethz.rosetta.versionExported
true
ethz.COinS
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