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dc.contributor.author
Senn, Maria M.
dc.contributor.author
Dengler, Vanina
dc.contributor.author
McCallum, Nadine
dc.contributor.author
Kiefer, Patrick
dc.contributor.author
Christen, Philipp
dc.contributor.author
Patrignani, Andrea
dc.contributor.author
Vorholt, Julia A.
dc.contributor.author
Berger-Bächi, Brigitte
dc.date.accessioned
2018-09-12T12:40:04Z
dc.date.available
2017-06-10T20:37:57Z
dc.date.available
2018-09-12T12:40:04Z
dc.date.issued
2013-08-27
dc.identifier.issn
1932-6203
dc.identifier.other
10.1371/journal.pone.0073512
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/70956
dc.identifier.doi
10.3929/ethz-b-000070956
dc.description.abstract
Faster growing and more virulent strains of methicillin resistant Staphylococcus aureus (MRSA) are increasingly displacing highly resistant MRSA. Elevated fitness in these MRSA is often accompanied by decreased and heterogeneous levels of methicillin resistance; however, the mechanisms for this phenomenon are not yet fully understood. Whole genome sequencing was used to investigate the genetic basis of this apparent correlation, in an isogenic MRSA strain pair that differed in methicillin resistance levels and fitness, with respect to growth rate. Sequencing revealed only one single nucleotide polymorphism (SNP) in the diadenylate cyclase gene dacA in the faster growing but less resistant strain. Diadenylate cyclases were recently discovered to synthesize the new second messenger cyclic diadenosine monophosphate (c-di-AMP). Introduction of this mutation into the highly resistant but slower growing strain reduced resistance and increased its growth rate, suggesting a direct connection between the dacA mutation and the phenotypic differences of these strains. Quantification of cellular c-di-AMP revealed that the dacA mutation decreased c-di-AMP levels resulting in reduced autolysis, increased salt tolerance and a reduction in the basal expression of the cell wall stress stimulon. These results indicate that c-di-AMP affects cell envelope-related signalling in S. aureus. The influence of c-di-AMP on growth rate and methicillin resistance in MRSA indicate that altering c-di-AMP levels could be a mechanism by which MRSA strains can increase their fitness levels by reducing their methicillin resistance levels.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Public Library of Science
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/3.0/
dc.title
Mutation in the C-Di-AMP Cyclase dacA Affects Fitness and Resistance of Methicillin Resistant Staphylococcus aureus
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 3.0 Unported
ethz.journal.title
PLoS ONE
ethz.journal.volume
8
en_US
ethz.journal.issue
8
en_US
ethz.journal.abbreviated
PLoS ONE
ethz.pages.start
e73512
en_US
ethz.size
11 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.nebis
006206116
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00003 - Schulleitung und Dienste::00022 - Bereich VP Forschung / Domain VP Research::02207 - Functional Genomics Center Zurich / Functional Genomics Center Zurich
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02520 - Institut für Mikrobiologie / Institute of Microbiology::03740 - Vorholt, Julia / Vorholt, Julia
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00003 - Schulleitung und Dienste::00022 - Bereich VP Forschung / Domain VP Research::02207 - Functional Genomics Center Zurich / Functional Genomics Center Zurich
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02520 - Institut für Mikrobiologie / Institute of Microbiology::03740 - Vorholt, Julia / Vorholt, Julia
ethz.date.deposited
2017-06-10T20:41:03Z
ethz.source
ECIT
ethz.identifier.importid
imp593650ec2ebfe35743
ethz.ecitpid
pub:112392
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-15T08:42:41Z
ethz.rosetta.lastUpdated
2022-03-28T21:18:11Z
ethz.rosetta.versionExported
true
ethz.COinS
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