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dc.contributor.author
Park, C. Sehwan
dc.contributor.author
Rehrauer, Hubert
dc.contributor.author
Mansuy, Isabelle M.
dc.date.accessioned
2018-10-09T11:58:21Z
dc.date.available
2017-06-10T20:46:33Z
dc.date.available
2018-10-09T11:58:21Z
dc.date.issued
2013-08
dc.identifier.other
10.1186/1471-2164-14-539
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/71121
dc.identifier.doi
10.3929/ethz-b-000071121
dc.description.abstract
Background Histone acetylation has been implicated in learning and memory in the brain, however, its function at the level of the genome and at individual genetic loci remains poorly investigated. This study examines a key acetylation mark, histone H4 lysine 5 acetylation (H4K5ac), genome-wide and its role in activity-dependent gene transcription in the adult mouse hippocampus following contextual fear conditioning. Results Using ChIP-Seq, we identified 23,235 genes in which H4K5ac correlates with absolute gene expression in the hippocampus. However, in the absence of transcription factor binding sites 150 bp upstream of the transcription start site, genes were associated with higher H4K5ac and expression levels. We further establish H4K5ac as a ubiquitous modification across the genome. Approximately one-third of all genes have above average H4K5ac, of which ~15% are specific to memory formation and ~65% are co-acetylated for H4K12. Although H4K5ac is prevalent across the genome, enrichment of H4K5ac at specific regions in the promoter and coding region are associated with different levels of gene expression. Additionally, unbiased peak calling for genes differentially acetylated for H4K5ac identified 114 unique genes specific to fear memory, over half of which have not previously been associated with memory processes. Conclusions Our data provide novel insights into potential mechanisms of gene priming and bookmarking by histone acetylation following hippocampal memory activation. Specifically, we propose that hyperacetylation of H4K5 may prime genes for rapid expression following activity. More broadly, this study strengthens the importance of histone posttranslational modifications for the differential regulation of transcriptional programs in cognitive processes.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
BioMed Central
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/2.0/
dc.subject
ChIP-Seq
en_US
dc.subject
Contextual fear conditioning
en_US
dc.subject
Gene bookmarking
en_US
dc.subject
Gene priming
en_US
dc.subject
H4K5 acetylation
en_US
dc.subject
Learning and memory
en_US
dc.title
Genome-wide analysis of H4K5 acetylation associated with fear memory in mice
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 2.0 Generic
ethz.journal.title
BMC Genomics
ethz.journal.volume
14
en_US
ethz.pages.start
539
en_US
ethz.size
19 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.nebis
004256340
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00003 - Schulleitung und Dienste::00022 - Bereich VP Forschung & Wirtschaftsbez. / Domain VP Research & Corporate Relations::02207 - Functional Genomics Center Zürich / Functional Genomics Center Zürich
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02542 - Institut für Neurowissenschaften / Institute for Neuroscience::03518 - Mansuy, Isabelle / Mansuy, Isabelle
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00003 - Schulleitung und Dienste::00022 - Bereich VP Forschung & Wirtschaftsbez. / Domain VP Research & Corporate Relations::02207 - Functional Genomics Center Zürich / Functional Genomics Center Zürich
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02542 - Institut für Neurowissenschaften / Institute for Neuroscience::03518 - Mansuy, Isabelle / Mansuy, Isabelle
ethz.date.deposited
2017-06-10T20:46:45Z
ethz.source
ECIT
ethz.identifier.importid
imp593650efdd18b73909
ethz.ecitpid
pub:112719
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-26T19:08:10Z
ethz.rosetta.lastUpdated
2018-10-09T11:58:25Z
ethz.rosetta.versionExported
true
ethz.COinS
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