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dc.contributor.author
Li, Bojun
dc.contributor.author
Moshfegh, Cameron
dc.contributor.author
Lin, Zhe
dc.contributor.author
Albuschies, Jörg
dc.contributor.author
Vogel, Viola
dc.date.accessioned
2018-09-10T14:36:22Z
dc.date.available
2017-06-10T20:49:09Z
dc.date.available
2018-09-10T14:36:22Z
dc.date.issued
2013-08-13
dc.identifier.other
10.1038/srep02425
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/71142
dc.identifier.doi
10.3929/ethz-b-000071142
dc.description.abstract
While stem cells can sense and respond to physical properties of their environment, the molecular aspects how physical information is translated into biochemical signals remain unknown. Here we show that human mesenchymal stem cells (hMSCs) harvest and assemble plasma fibronectin into their extracellular matrix (ECM) fibrils within 24 hours. hMSCs pro-actively pull on newly assembled fibronectin ECM fibrils, and the fibers are more stretched on rigid than on soft fibronectin-coated polyacrylamide gels. Culturing hMSCs on single stretched fibronectin fibers upregulates hMSC osteogenesis. Osteogenesis was increased when αvβ3 integrins were blocked on relaxed fibronectin fibers, and decreased when α5β1 integrins were blocked or when epidermal growth factor (EGF) receptor signaling was inhibited on stretched fibronectin fibers. This suggests that hMSCs utilize their own contractile forces to translate environmental cues into differential biochemical signals by stretching fibronectin fibrils. Mechanoregulation of fibronectin fibrils may thus serve as check point to regulate hMSC osteogenesis.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature Publishing Group
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/3.0/
dc.subject
Biophysics
en_US
dc.subject
Biotechnology
en_US
dc.subject
Cell Biology
en_US
dc.subject
Stem Cells
en_US
dc.subject
Subject Areas
en_US
dc.title
Mesenchymal Stem Cells Exploit Extracellular Matrix as Mechanotransducer
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 3.0 Unported
ethz.journal.title
Scientific Reports
ethz.journal.volume
3
en_US
ethz.pages.start
2425
en_US
ethz.size
8 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.identifier.nebis
006751867
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02540 - Institut für Translationale Medizin / Institute of Translational Medicine::03640 - Vogel, Viola / Vogel, Viola
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02540 - Institut für Translationale Medizin / Institute of Translational Medicine::03640 - Vogel, Viola / Vogel, Viola
ethz.date.deposited
2017-06-10T20:52:34Z
ethz.source
ECIT
ethz.identifier.importid
imp593650f066bdd44398
ethz.ecitpid
pub:112753
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-19T11:53:35Z
ethz.rosetta.lastUpdated
2019-01-02T13:51:33Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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