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dc.contributor.author
Handke, Björn
dc.contributor.author
Poernbacher, Ingrid
dc.contributor.author
Goetze, Sandra
dc.contributor.author
Ahrens, Christian H.
dc.contributor.author
Omasits, Ulrich
dc.contributor.author
Marty, Florian
dc.contributor.author
Simigdala, Nikiana
dc.contributor.author
Meyer, Imke
dc.contributor.author
Wollscheid, Bernd
dc.contributor.author
Brunner, Erich
dc.contributor.author
Hafen, Ernst
dc.contributor.author
Lehner, Christian F.
dc.date.accessioned
2018-09-12T12:51:31Z
dc.date.available
2017-06-10T21:16:15Z
dc.date.available
2018-09-12T12:51:31Z
dc.date.issued
2013-06-20
dc.identifier.issn
1932-6203
dc.identifier.other
10.1371/journal.pone.0067208
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/71628
dc.identifier.doi
10.3929/ethz-b-000071628
dc.description.abstract
The co-operation of specialized organ systems in complex multicellular organisms depends on effective chemical communication. Thus, body fluids (like blood, lymph or intraspinal fluid) contain myriads of signaling mediators apart from metabolites. Moreover, these fluids are also of crucial importance for immune and wound responses. Compositional analyses of human body fluids are therefore of paramount diagnostic importance. Further improving their comprehensiveness should increase our understanding of inter-organ communication. In arthropods, which have trachea for gas exchange and an open circulatory system, the single dominating interstitial fluid is the hemolymph. Accordingly, a detailed analysis of hemolymph composition should provide an especially comprehensive picture of chemical communication and defense in animals. Therefore we used an extensive protein fractionation workflow in combination with a discovery-driven proteomic approach to map out the detectable protein composition of hemolymph isolated from Drosophila larvae. Combined mass spectrometric analysis revealed more than 700 proteins extending far beyond the previously known Drosophila hemolymph proteome. Moreover, by comparing hemolymph isolated from either fed or starved larvae, we provide initial provisional insights concerning compositional changes in response to nutritional state. Storage proteins in particular were observed to be strongly reduced by starvation. Our hemolymph proteome catalog provides a rich basis for data mining, as exemplified by our identification of potential novel cytokines, as well as for future quantitative analyses by targeted proteomics.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Public Library Science
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/3.0/
dc.title
The Hemolymph Proteome of Fed and Starved Drosophila Larvae
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 3.0 Unported
ethz.journal.title
PLoS ONE
ethz.journal.volume
8
en_US
ethz.journal.issue
6
en_US
ethz.journal.abbreviated
PLoS ONE
ethz.pages.start
e67208
en_US
ethz.size
10 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.nebis
006206116
ethz.publication.place
San Francisco, CA, USA
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::03710 - Hafen, Ernst (emeritus) / Hafen, Ernst (emeritus)
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::03710 - Hafen, Ernst (emeritus) / Hafen, Ernst (emeritus)
ethz.date.deposited
2017-06-10T21:19:23Z
ethz.source
ECIT
ethz.identifier.importid
imp593650fb92ea093662
ethz.ecitpid
pub:113521
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-17T09:02:03Z
ethz.rosetta.lastUpdated
2022-03-28T21:18:12Z
ethz.rosetta.versionExported
true
ethz.COinS
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