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dc.contributor.author
Möller, Jens
dc.contributor.author
Lühmann, Tessa
dc.contributor.author
Chabria, Mamta
dc.contributor.author
Hall, Heike
dc.contributor.author
Vogel, Viola
dc.date.accessioned
2018-09-05T14:23:12Z
dc.date.available
2017-06-10T22:01:41Z
dc.date.available
2018-09-05T14:23:12Z
dc.date.issued
2013-10-07
dc.identifier.other
10.1038/srep02884
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/72468
dc.identifier.doi
10.3929/ethz-b-000072468
dc.description.abstract
To clear pathogens from host tissues or biomaterial surfaces, phagocytes have to break the adhesive bacteria-substrate interactions. Here we analysed the mechanobiological process that enables macrophages to lift-off and phagocytose surface-bound Escherichia coli (E. coli). In this opsonin-independent process, macrophage filopodia hold on to the E. coli fimbriae long enough to induce a local protrusion of a lamellipodium. Specific contacts between the macrophage and E. coli are formed via the glycoprotein CD48 on filopodia and the adhesin FimH on type 1 fimbriae (hook). We show that bacterial detachment from surfaces occurrs after a lamellipodium has protruded underneath the bacterium (shovel), thereby breaking the multiple bacterium-surface interactions. After lift-off, the bacterium is engulfed by a phagocytic cup. Force activated catch bonds enable the long-term survival of the filopodium-fimbrium interactions while soluble mannose inhibitors and CD48 antibodies suppress the contact formation and thereby inhibit subsequent E. coli phagocytosis.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature Publishing Group
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/3.0/
dc.subject
Filopodia
en_US
dc.subject
Infection
en_US
dc.subject
Lamellipodia
en_US
dc.subject
Phagocytes
en_US
dc.title
Macrophages lift off surface-bound bacteria using a filopodium-lamellipodium hook-and-shovel mechanism
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 3.0 Unported
ethz.journal.title
Scientific Reports
ethz.journal.volume
3
en_US
ethz.pages.start
2884
en_US
ethz.size
8 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.scopus
ethz.identifier.scopus
ethz.identifier.nebis
006751867
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02540 - Institut für Translationale Medizin / Institute of Translational Medicine::03640 - Vogel, Viola / Vogel, Viola
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02540 - Institut für Translationale Medizin / Institute of Translational Medicine::03640 - Vogel, Viola / Vogel, Viola
ethz.date.deposited
2017-06-10T22:01:59Z
ethz.source
ECIT
ethz.identifier.importid
imp5936510c103f591536
ethz.ecitpid
pub:114803
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-19T12:15:37Z
ethz.rosetta.lastUpdated
2019-01-02T13:44:50Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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