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dc.contributor.author
Weissbrodt, David G.
dc.contributor.author
Neu, Thomas R.
dc.contributor.author
Kuhlicke, Ute
dc.contributor.author
Rappaz, Yoan
dc.contributor.author
Holliger, Christof
dc.date.accessioned
2018-11-05T14:08:21Z
dc.date.available
2017-06-10T22:09:59Z
dc.date.available
2018-11-05T14:08:21Z
dc.date.issued
2013-07-10
dc.identifier.issn
1664-302X
dc.identifier.other
10.3389/fmicb.2013.00175
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/72627
dc.identifier.doi
10.3929/ethz-b-000072627
dc.description.abstract
Aerobic granular sludge (AGS) is based on self-granulated flocs forming mobile biofilms with a gel-like consistence. Bacterial and structural dynamics from flocs to granules were followed in anaerobic-aerobic sequencing batch reactors (SBR) fed with synthetic wastewater, namely a bubble column (BC-SBR) operated under wash-out conditions for fast granulation, and two stirred-tank enrichments of Accumulibacter (PAO-SBR) and Competibacter (GAO-SBR) operated at steady-state. In the BC-SBR, granules formed within 2 weeks by swelling of Zoogloea colonies around flocs, developing subsequently smooth zoogloeal biofilms. However, Zoogloea predominance (37–79%) led to deteriorated nutrient removal during the first months of reactor operation. Upon maturation, improved nitrification (80–100%), nitrogen removal (43–83%), and high but unstable dephosphatation (75–100%) were obtained. Proliferation of dense clusters of nitrifiers, Accumulibacter, and Competibacter from granule cores outwards resulted in heterogeneous bioaggregates, inside which only low abundance Zoogloea (<5%) were detected in biofilm interstices. The presence of different extracellular glycoconjugates detected by fluorescence lectin-binding analysis showed the complex nature of the intracellular matrix of these granules. In the PAO-SBR, granulation occurred within two months with abundant and active Accumulibacter populations (56 ± 10%) that were selected under full anaerobic uptake of volatile fatty acids and that aggregated as dense clusters within heterogeneous granules. Flocs self-granulated in the GAO-SBR after 480 days during a period of over-aeration caused by biofilm growth on the oxygen sensor. Granules were dominated by heterogeneous clusters of Competibacter (37 ± 11%). Zoogloea were never abundant in biomass of both PAO- and GAO-SBRs. This study showed that Zoogloea, Accumulibacter, and Competibacter affiliates can form granules, and that the granulation mechanisms rely on the dominant population involved.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Frontiers Research Foundation
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/3.0/
dc.subject
Biological wastewater treatment
en_US
dc.subject
Aerobic granular sludge
en_US
dc.subject
Granular biofilm formation and structure
en_US
dc.subject
T-RFLP
en_US
dc.subject
Pyrosequencing
en_US
dc.subject
CLSM
en_US
dc.subject
FISH
en_US
dc.subject
FLBA
en_US
dc.title
Assessment of bacterial and structural dynamics in aerobic granular biofilms
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 3.0 Unported
ethz.journal.title
Frontiers in Microbiology
ethz.journal.volume
4
en_US
ethz.journal.abbreviated
Front Microbiol
ethz.pages.start
175
en_US
ethz.size
18 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Lausanne
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02115 - Dep. Bau, Umwelt und Geomatik / Dep. of Civil, Env. and Geomatic Eng.::02608 - Institut für Umweltingenieurwiss. / Institute of Environmental Engineering::03832 - Morgenroth, Eberhard / Morgenroth, Eberhard
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02115 - Dep. Bau, Umwelt und Geomatik / Dep. of Civil, Env. and Geomatic Eng.::02608 - Institut für Umweltingenieurwiss. / Institute of Environmental Engineering::03832 - Morgenroth, Eberhard / Morgenroth, Eberhard
ethz.date.deposited
2017-06-10T22:12:40Z
ethz.source
ECIT
ethz.identifier.importid
imp5936510e81f0573115
ethz.ecitpid
pub:115091
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-13T13:24:05Z
ethz.rosetta.lastUpdated
2022-03-28T21:35:09Z
ethz.rosetta.versionExported
true
ethz.COinS
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