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dc.contributor.author
Occhipinti, Laura
dc.contributor.author
Chang, Yiming
dc.contributor.author
Altvater, Martin
dc.contributor.author
Menet, Anna M.
dc.contributor.author
Kemmler, Stefan
dc.contributor.author
Panse, Vikram G.
dc.date.accessioned
2019-04-02T16:13:00Z
dc.date.available
2017-06-10T22:29:20Z
dc.date.available
2019-04-02T16:13:00Z
dc.date.issued
2013-09
dc.identifier.other
10.1093/nar/gkt675
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/72946
dc.identifier.doi
10.3929/ethz-b-000072946
dc.description.abstract
Multiple export receptors passage bound pre-ribosomes through nuclear pore complexes (NPCs) by transiently interacting with the Phe-Gly (FG) meshwork of their transport channels. Here, we reveal how the non-FG interacting yeast mRNA export factor Gly-Leu-FG lethal 2 (Gle2) functions in the export of the large pre-ribosomal subunit (pre-60S). Structure-guided studies uncovered conserved platforms used by Gle2 to export pre-60S: an uncharacterized basic patch required to bind pre-60S, and a second surface that makes non-FG contacts with the nucleoporin Nup116. A basic patch mutant of Gle2 is able to function in mRNA export, but not pre-60S export. Thus, Gle2 provides a distinct interaction platform to transport pre-60S to the cytoplasm. Notably, Gle2’s interaction platforms become crucial for pre-60S export when FG-interacting receptors are either not recruited to pre-60S or are impaired. We propose that large complex cargos rely on non-FG as well as FG-interactions for their efficient translocation through the nuclear pore complex channel.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Oxford University Press
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/3.0/
dc.title
Non-FG mediated transport of the large pre-ribosomal subunit through the nuclear pore complex by the mRNA export factor Gle2
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 3.0 Unported
dc.date.published
2013-07-31
ethz.journal.title
Nucleic acids research
ethz.journal.volume
41
en_US
ethz.journal.issue
17
en_US
ethz.pages.start
8266
en_US
ethz.pages.end
8279
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.nebis
000038633
ethz.publication.place
Oxford
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich, direkt::00012 - Lehre und Forschung, direkt::00007 - Departemente, direkt::02030 - Departement Biologie / Department of Biology::02517 - Institut für Biochemie (IBC) / Institute of Biochemistry (IBC)::03884 - Panse, Vikram G. (SNF-Professur) (ehemalig)
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich, direkt::00012 - Lehre und Forschung, direkt::00007 - Departemente, direkt::02030 - Departement Biologie / Department of Biology::02517 - Institut für Biochemie (IBC) / Institute of Biochemistry (IBC)::03884 - Panse, Vikram G. (SNF-Professur) (ehemalig)
ethz.date.deposited
2017-06-10T22:31:00Z
ethz.source
ECIT
ethz.identifier.importid
imp593651121f3c217799
ethz.ecitpid
pub:115562
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-14T18:16:29Z
ethz.rosetta.lastUpdated
2019-04-02T16:13:43Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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