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dc.contributor.author
Paris, Margot
dc.contributor.author
Marcombe, Sebastien
dc.contributor.author
Coissac, Eric
dc.contributor.author
Corbel, Vincent
dc.contributor.author
David, Jean-Philippe
dc.contributor.author
Després, Laurence
dc.date.accessioned
2019-07-02T13:26:27Z
dc.date.available
2017-06-10T22:54:56Z
dc.date.available
2019-07-02T13:26:27Z
dc.date.issued
2013-11
dc.identifier.issn
1752-4571
dc.identifier.issn
1752-4563
dc.identifier.other
10.1111/eva.12082
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/73431
dc.identifier.doi
10.3929/ethz-b-000073431
dc.description.abstract
Mosquito control is often the main method used to reduce mosquito‐transmitted diseases. In order to investigate the genetic basis of resistance to the bio‐insecticide Bacillus thuringiensis subsp. israelensis (Bti), we used information on polymorphism obtained from cDNA tag sequences from pooled larvae of laboratory Bti‐resistant and susceptible Aedes aegypti mosquito strains to identify and analyse 1520 single nucleotide polymorphisms (SNPs). Of the 372 SNPs tested, 99.2% were validated using DNA Illumina GoldenGate® array, with a strong correlation between the allelic frequencies inferred from the pooled and individual data (r = 0.85). A total of 11 genomic regions and five candidate genes were detected using a genome scan approach. One of these candidate genes showed significant departures from neutrality in the resistant strain at sequence level. Six natural populations from Martinique Island were sequenced for the 372 tested SNPs with a high transferability (87%), and association mapping analyses detected 14 loci associated with Bti resistance, including one located in a putative receptor for Cry11 toxins. Three of these loci were also significantly differentiated between the laboratory strains, suggesting that most of the genes associated with resistance might differ between the two environments. It also suggests that common selected regions might harbour key genes for Bti resistance.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Wiley
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/3.0/
dc.subject
Aedes aegypti
en_US
dc.subject
Bacillus thurigiensis var. israelensis
en_US
dc.subject
genome scan
en_US
dc.subject
insecticide resistance
en_US
dc.subject
next‐generation sequencing
en_US
dc.subject
RNAseq
en_US
dc.title
Investigating the genetics of Bti resistance using mRNA tag sequencing: application on laboratory strains and natural populations of the dengue vector Aedes aegypti
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 3.0 Unported
dc.date.published
2013-08-31
ethz.journal.title
Evolutionary Applications
ethz.journal.volume
6
en_US
ethz.journal.issue
7
en_US
ethz.journal.abbreviated
Evol. appl.
ethz.pages.start
1012
en_US
ethz.pages.end
1027
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.nebis
006994734
ethz.publication.place
S.l.
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02720 - Institut für Integrative Biologie / Institute of Integrative Biology::03706 - Widmer, Alexander / Widmer, Alexander
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02720 - Institut für Integrative Biologie / Institute of Integrative Biology::03706 - Widmer, Alexander / Widmer, Alexander
ethz.date.deposited
2017-06-10T22:56:16Z
ethz.source
ECIT
ethz.identifier.importid
imp5936511b77fe442597
ethz.ecitpid
pub:116346
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-13T11:20:40Z
ethz.rosetta.lastUpdated
2019-07-02T13:26:37Z
ethz.rosetta.versionExported
true
ethz.COinS
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