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dc.contributor.author
Rühs, Patrick A.
dc.contributor.author
Böni, Lukas
dc.contributor.author
Fuller, Gerald G.
dc.contributor.author
Inglis, R. Fredrik
dc.contributor.author
Fischer, Peter
dc.date.accessioned
2018-09-06T14:07:13Z
dc.date.available
2017-06-10T23:10:42Z
dc.date.available
2018-09-06T14:07:13Z
dc.date.issued
2013-11-11
dc.identifier.issn
1932-6203
dc.identifier.other
10.1371/journal.pone.0078524
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/73691
dc.identifier.doi
10.3929/ethz-b-000073691
dc.description.abstract
Understanding the numerous factors that can affect biofilm formation and stability remain poorly understood. One of the major limitations is the accurate measurement of biofilm stability and cohesiveness in real-time when exposed to changing environmental conditions. Here we present a novel method to measure biofilm strength: interfacial rheology. By culturing a range of bacterial biofilms on an air-liquid interface we were able to measure their viscoelastic growth profile during and after biofilm formation and subsequently alter growth conditions by adding surfactants or changing the nutrient composition of the growth medium. We found that different bacterial species had unique viscoelastic growth profiles, which was also highly dependent on the growth media used. We also found that we could reduce biofilm formation by the addition of surfactants or changing the pH, thereby altering the viscoelastic properties of the biofilm. Using this technique we were able to monitor changes in viscosity, elasticity and surface tension online, under constant and varying environmental conditions, thereby providing a complementary method to better understand the dynamics of both biofilm formation and dispersal.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Public Library of Science
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/3.0/
dc.title
In-Situ Quantification of the Interfacial Rheological Response of Bacterial Biofilms to Environmental Stimuli
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 3.0 Unported
ethz.journal.title
PLoS ONE
ethz.journal.volume
8
en_US
ethz.journal.issue
11
en_US
ethz.journal.abbreviated
PLoS ONE
ethz.pages.start
e78524
en_US
ethz.size
9 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.scopus
ethz.identifier.nebis
006206116
ethz.publication.place
Lawrence, KS, USA
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02701 - Inst.f. Lebensmittelwiss.,Ernährung,Ges. / Institute of Food, Nutrition, and Health::03345 - Windhab, Erich Josef / Windhab, Erich Josef::08821 - Fischer, Peter (Tit.-Prof.)
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02721 - Inst. f. Biogeochemie u. Schadstoffdyn. / Inst. Biogeochem. and Pollutant Dynamics::03743 - Ackermann, Martin / Ackermann, Martin
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02701 - Inst.f. Lebensmittelwiss.,Ernährung,Ges. / Institute of Food, Nutrition, and Health::03345 - Windhab, Erich Josef / Windhab, Erich Josef
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02701 - Inst.f. Lebensmittelwiss.,Ernährung,Ges. / Institute of Food, Nutrition, and Health::03345 - Windhab, Erich Josef / Windhab, Erich Josef::08821 - Fischer, Peter (Tit.-Prof.)
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02721 - Inst. f. Biogeochemie u. Schadstoffdyn. / Inst. Biogeochem. and Pollutant Dynamics::03743 - Ackermann, Martin / Ackermann, Martin
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02701 - Inst.f. Lebensmittelwiss.,Ernährung,Ges. / Institute of Food, Nutrition, and Health::03345 - Windhab, Erich Josef / Windhab, Erich Josef
ethz.date.deposited
2017-06-10T23:12:58Z
ethz.source
ECIT
ethz.identifier.importid
imp5936512126ff926370
ethz.ecitpid
pub:116644
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-26T03:49:41Z
ethz.rosetta.lastUpdated
2018-11-08T01:42:46Z
ethz.rosetta.versionExported
true
ethz.COinS
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