High-resolution imaging of 2D outer membrane protein F crystals by atomic force microscopy
- Book Chapter
In this chapter the methodological bases are provided to achieve subnanometer resolution on two-dimensional (2D) membrane protein crystals by atomic force microscopy (AFM). This is outlined in detail with the example of AFM studies of the outer membrane protein F (OmpF) from the bacterium Escherichia coli (E. coli). We describe in detail the high-resolution imaging of 2D OmpF crystals in aqueous solution and under near-physiological conditions. The topographs of OmpF, and stylus effects and artifacts encountered when imaging by AFM are discussed. Show more
Book titleElectron Cystallography of Soluble and Membrane Proteins
Journal / seriesMethods in Molecular Biology
Pages / Article No.
SubjectAtomic force microscopy; Biological membranes; High resolution; Outer membrane protein F; Stylus artifacts; Topography
Organisational unit03870 - Müller, Daniel J. / Müller, Daniel J.
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