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dc.contributor.author
Chubukov, Victor
dc.contributor.author
Uhr, Markus
dc.contributor.author
Le Chat, Ludovic
dc.contributor.author
Kleijn, Roelco J.
dc.contributor.author
Jules, Matthieu
dc.contributor.author
Link, Hannes
dc.contributor.author
Aymerich, Stephane
dc.contributor.author
Stelling, Jörg
dc.contributor.author
Sauer, Uwe
dc.date.accessioned
2018-06-07T15:25:57Z
dc.date.available
2017-06-11T02:37:33Z
dc.date.available
2018-06-07T15:25:57Z
dc.date.issued
2013-01
dc.identifier.issn
1744-4292
dc.identifier.other
10.1038/msb.2013.66
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/77583
dc.identifier.doi
10.3929/ethz-b-000077583
dc.description.abstract
One of the key ways in which microbes are thought to regulate their metabolism is by modulating the availability of enzymes through transcriptional regulation. However, the limited success of efforts to manipulate metabolic fluxes by rewiring the transcriptional network has cast doubt on the idea that transcript abundance controls metabolic fluxes. In this study, we investigate control of metabolic flux in the model bacterium Bacillus subtilis by quantifying fluxes, transcripts, and metabolites in eight metabolic states enforced by different environmental conditions. We find that most enzymes whose flux switches between on and off states, such as those involved in substrate uptake, exhibit large corresponding transcriptional changes. However, for the majority of enzymes in central metabolism, enzyme concentrations were insufficient to explain the observed fluxes—only for a number of reactions in the tricarboxylic acid cycle were enzyme changes approximately proportional to flux changes. Surprisingly, substrate changes revealed by metabolomics were also insufficient to explain observed fluxes, leaving a large role for allosteric regulation and enzyme modification in the control of metabolic fluxes.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature
dc.rights.uri
http://creativecommons.org/licenses/by-nc-sa/3.0/
dc.subject
Central carbon metabolism
en_US
dc.subject
Metabolic flux
en_US
dc.subject
Transcriptional regulation
en_US
dc.title
Transcriptional regulation is insufficient to explain substrate‐induced flux changes in Bacillus subtilis
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported
dc.date.published
2013-11-26
ethz.journal.title
Molecular Systems Biology
ethz.journal.volume
9
en_US
ethz.journal.issue
1
en_US
ethz.journal.abbreviated
Mol Syst Biol
ethz.pages.start
709
en_US
ethz.size
13 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.nebis
004931271
ethz.publication.place
London
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::03699 - Stelling, Jörg / Stelling, Jörg
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::03713 - Sauer, Uwe / Sauer, Uwe
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::03699 - Stelling, Jörg / Stelling, Jörg
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::03713 - Sauer, Uwe / Sauer, Uwe
ethz.date.deposited
2017-06-11T02:37:39Z
ethz.source
ECIT
ethz.identifier.importid
imp5936516c6d3b834364
ethz.ecitpid
pub:122256
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-15T10:20:01Z
ethz.rosetta.lastUpdated
2024-02-02T05:01:32Z
ethz.rosetta.versionExported
true
ethz.COinS
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