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dc.contributor.author
Al Fahad, Ahmed
dc.contributor.author
Abood, Amira
dc.contributor.author
Fisch, Katja M.
dc.contributor.author
Osipow, Anna
dc.contributor.author
Davison, Jack
dc.contributor.author
Avramovic, Marija
dc.contributor.author
Butts, Craig P.
dc.contributor.author
Piel, Jörn
dc.contributor.author
Simpson, Thomas J.
dc.contributor.author
Cox, Russell J.
dc.date.accessioned
2019-06-06T10:48:44Z
dc.date.available
2017-06-11T03:11:22Z
dc.date.available
2019-06-06T10:48:44Z
dc.date.issued
2014-02
dc.identifier.issn
2041-6520
dc.identifier.issn
2041-6539
dc.identifier.other
10.1039/c3sc52911h
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/78290
dc.identifier.doi
10.3929/ethz-b-000078290
dc.description.abstract
An FAD-dependent monooxygenase encoding gene (SorbC) was cloned from Penicillium chrysogenum E01-10/3 and expressed as a soluble protein in Escherichia coli. The enzyme efficiently performed the oxidative dearomatisation of sorbicillin and dihydrosorbicillin to give sorbicillinol and dihydrosorbicillinol respectively. Bioinformatic examination of the gene cluster surrounding SorbC indicated the presence of two polyketide synthase (PKS) encoding genes designated sorbA and sorbB. The gene sorbA-encodes a highly reducing iterative PKS while SorbB encodes a non-reducing iterative PKS which features a reductive release domain usually involved in the production of polyketide aldehydes. Using these observations and previously reported results from isotopic feeding experiments a new and simpler biosynthetic route to the sorbicillin class of secondary metabolites is proposed which is consistent with all reported experimental results.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Royal Society of Chemistry
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/3.0/
dc.title
Oxidative dearomatisation: the key step of sorbicillinoid biosynthesis
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 3.0 Unported
dc.date.published
2013-11-20
ethz.journal.title
Chemical Science
ethz.journal.volume
5
en_US
ethz.journal.issue
2
en_US
ethz.journal.abbreviated
Chem. sci.
ethz.pages.start
523
en_US
ethz.pages.end
527
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.identifier.nebis
006104946
ethz.publication.place
Cambridge
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02520 - Institut für Mikrobiologie / Institute of Microbiology::03980 - Piel, Jörn / Piel, Jörn
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02520 - Institut für Mikrobiologie / Institute of Microbiology::03980 - Piel, Jörn / Piel, Jörn
ethz.date.deposited
2017-06-11T03:14:17Z
ethz.source
ECIT
ethz.identifier.importid
imp59365179432a422879
ethz.ecitpid
pub:123244
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-14T12:03:14Z
ethz.rosetta.lastUpdated
2019-06-06T10:48:52Z
ethz.rosetta.versionExported
true
ethz.COinS
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