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dc.contributor.author
Katsuyama, Tomonori
dc.contributor.author
Akmammedov, Arslan
dc.contributor.author
Seimiya, Makiko
dc.contributor.author
Hess, Samuel C.
dc.contributor.author
Sievers, Cem
dc.contributor.author
Paro, Renato
dc.date.accessioned
2019-04-02T16:38:00Z
dc.date.available
2017-06-11T04:17:07Z
dc.date.available
2019-04-02T16:38:00Z
dc.date.issued
2013-09
dc.identifier.issn
1362-4962
dc.identifier.issn
0305-1048
dc.identifier.issn
1362-4954
dc.identifier.other
10.1093/nar/gkt638
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/79595
dc.identifier.doi
10.3929/ethz-b-000079595
dc.description.abstract
In reverse genetics, a gene’s function is elucidated through targeted modifications in the coding region or associated DNA cis -regulatory elements. To this purpose, recently developed customizable transcription activator-like effector nucleases (TALENs) have proven an invaluable tool, allowing introduction of double-strand breaks at predetermined sites in the genome. Here we describe a practical and efficient method for the targeted genome engineering in Drosophila . We demonstrate TALEN-mediated targeted gene integration and efficient identification of mutant flies using a traceable marker phenotype. Furthermore, we developed an easy TALEN assembly (easyT) method relying on simultaneous reactions of DNA Bae I digestion and ligation, enabling construction of complete TALENs from a monomer unit library in a single day. Taken together, our strategy with easyT and TALEN-plasmid microinjection simplifies mutant generation and enables isolation of desired mutant fly lines in the F 1 generation.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Oxford University Press
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc/3.0/
dc.title
An efficient strategy for TALEN-mediated genome engineering in Drosophila
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial 3.0 Unported
dc.date.published
2013-07-22
ethz.journal.title
Nucleic Acids Research
ethz.journal.volume
41
en_US
ethz.journal.issue
17
en_US
ethz.journal.abbreviated
Nucleic Acids Res.
ethz.pages.start
e163
en_US
ethz.size
9 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.nebis
000038633
ethz.publication.place
Oxford
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::03748 - Paro, Renato / Paro, Renato
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::03748 - Paro, Renato / Paro, Renato
ethz.date.deposited
2017-06-11T04:17:56Z
ethz.source
ECIT
ethz.identifier.importid
imp59365190c752664798
ethz.ecitpid
pub:125002
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-31T15:35:29Z
ethz.rosetta.lastUpdated
2019-04-02T16:38:39Z
ethz.rosetta.exportRequired
false
ethz.rosetta.versionExported
true
ethz.COinS
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