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dc.contributor.author
Steffen, Philipp A.
dc.contributor.author
Fonseca, João Pedro
dc.contributor.author
Gänger, Cornelia
dc.contributor.author
Dworschak, Eva
dc.contributor.author
Kockmann, Tobias
dc.contributor.author
Beisel, Christian
dc.contributor.author
Ringrose, Leonie
dc.date.accessioned
2019-04-02T16:44:33Z
dc.date.available
2017-06-11T04:17:13Z
dc.date.available
2019-04-02T16:44:33Z
dc.date.issued
2013-05
dc.identifier.other
10.1093/nar/gkt217
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/79597
dc.identifier.doi
10.3929/ethz-b-000079597
dc.description.abstract
The Polycomb (PcG) and Trithorax (TrxG) group proteins work antagonistically on several hundred developmentally important target genes, giving stable mitotic memory, but also allowing flexibility of gene expression states. How this is achieved in quantitative terms is poorly understood. Here, we present a quantitative kinetic analysis in living Drosophila of the PcG proteins Enhancer of Zeste, (E(Z)), Pleiohomeotic (PHO) and Polycomb (PC) and the TrxG protein absent, small or homeotic discs 1 (ASH1). Fluorescence recovery after photobleaching and fluorescence correlation spectroscopy reveal highly dynamic chromatin binding behaviour for all proteins, with exchange occurring within seconds. We show that although the PcG proteins substantially dissociate from mitotic chromatin, ASH1 remains robustly associated with chromatin throughout mitosis. Finally, we show that chromatin binding by ASH1 and PC switches from an antagonistic relationship in interphase, to a cooperative one during mitosis. These results provide quantitative insights into PcG and TrxG chromatin-binding dynamics and have implications for our understanding of the molecular nature of epigenetic memory.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Oxford University Press
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc/3.0/
dc.title
Quantitative in vivo analysis of chromatin binding of Polycomb and Trithorax group proteins reveals retention of ASH1 on mitotic chromatin
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial 3.0 Unported
dc.date.published
2013-04-10
ethz.journal.title
Nucleic acids research
ethz.journal.volume
41
en_US
ethz.journal.issue
10
en_US
ethz.pages.start
5235
en_US
ethz.pages.end
5250
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.nebis
000038633
ethz.publication.place
Oxford
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::03748 - Paro, Renato (emeritus) / Paro, Renato (emeritus)
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::03748 - Paro, Renato (emeritus) / Paro, Renato (emeritus)
ethz.date.deposited
2017-06-11T04:17:56Z
ethz.source
ECIT
ethz.identifier.importid
imp59365190d1cda13264
ethz.ecitpid
pub:125004
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-26T19:13:31Z
ethz.rosetta.lastUpdated
2019-04-02T16:45:06Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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