A mass spectrometry-based hybrid method for structural modeling of protein complexes
- Journal Article
We describe a method that integrates data derived from different mass spectrometry (MS)-based techniques with a modeling strategy for structural characterization of protein assemblies. We encoded structural data derived from native MS, bottom-up proteomics, ion mobility–MS and chemical cross-linking MS into modeling restraints to compute the most likely structure of a protein assembly. We used the method to generate near-native models for three known structures and characterized an assembly intermediate of the proteasomal base. Show more
Journal / seriesNature Methods
Pages / Article No.
PublisherNature Publishing Group
Organisational unit03663 - Aebersold, Rudolf (emeritus) / Aebersold, Rudolf (emeritus)
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