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dc.contributor.author
Roesslein, Matthias
dc.contributor.author
Hirsch, Cordula
dc.contributor.author
Kaiser, Jean-Pierre
dc.contributor.author
Krug, Harald F.
dc.contributor.author
Wick, Peter
dc.date.accessioned
2018-11-05T11:00:17Z
dc.date.available
2017-06-11T05:15:16Z
dc.date.available
2018-11-05T11:00:17Z
dc.date.issued
2013-12-13
dc.identifier.issn
1422-0067
dc.identifier.other
10.3390/ijms141224320
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/80810
dc.identifier.doi
10.3929/ethz-b-000080810
dc.description.abstract
The release of reactive oxygen species (ROS) during the electron transport of mitochondrial aerobic respiration is the major source of ROS. However, contact between cells and nanoparticles (NPs) can also induce release of ROS, leading to an imbalance towards the pro-oxidative state. At low levels of ROS production, cells initiate a protective response to guarantee their survival, but an excess of ROS can damage cellular compounds such as membranes and various organelles, or directly cause genotoxicity. Thus an elevated level of ROS is an important indicator of cellular stress and an accurate recording of this parameter would be very informative. ROS can be measured by various assays, but all known assays measuring and quantifying ROS possess certain weaknesses. The problems and challenges of quantitatively detecting ROS in vitro using the 2',7'-dichlorodihydrofluorescein (DCF) assay is discussed as an example. In addition, we debate the difficulties in finding a suitable and stable chemical reaction control for the DCF assay (or other ROS-detecting assays). As a conclusion, we believe that using 3-morpholinosydnonimine hydrochloride (Sin-1) as a ROS inducer in the DCF assay is feasible only qualitatively. However, a quantitative measurement of the absolute amount of ROS produced and a quantitative comparison between experiments is (at the moment) impossible.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
MDPI
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/3.0/
dc.subject
Chemical reaction control
en_US
dc.subject
Comparability
en_US
dc.subject
DCF assay
en_US
dc.subject
Interference
en_US
dc.subject
Nanoparticles
en_US
dc.subject
Reactive oxygen species (ROS)
en_US
dc.subject
Sin-1
en_US
dc.title
Comparability of in Vitro Tests for Bioactive Nanoparticles: A Common Assay to Detect Reactive Oxygen Species as an Example
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 3.0 Unported
ethz.journal.title
International Journal of Molecular Sciences
ethz.journal.volume
14
en_US
ethz.journal.issue
12
en_US
ethz.journal.abbreviated
Int. j. mol. sci.
ethz.pages.start
24320
en_US
ethz.pages.end
24337
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.nebis
004068146
ethz.publication.place
S.l.
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2017-06-11T05:15:36Z
ethz.source
ECIT
ethz.identifier.importid
imp593651a65d51226541
ethz.ecitpid
pub:126939
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-13T13:34:51Z
ethz.rosetta.lastUpdated
2018-11-05T11:00:24Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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