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dc.contributor.author
Snedeker, Jess Gerrit
dc.contributor.author
Cadby, Jennifer A.
dc.contributor.author
Buehler, Evelyne
dc.contributor.author
Godbout, Charles
dc.contributor.author
van Weeren, P. Rene
dc.date.accessioned
2018-08-09T12:20:12Z
dc.date.available
2017-06-11T07:15:50Z
dc.date.available
2018-08-09T12:20:12Z
dc.date.issued
2014-03-20
dc.identifier.issn
1932-6203
dc.identifier.other
10.1371/journal.pone.0092474
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/82833
dc.identifier.doi
10.3929/ethz-b-000082833
dc.description.abstract
The role of intrinsic and extrinsic healing in injured tendons is still debated. In this study, we characterized cell plasticity, proliferative capacity, and migration characteristics as proxy measures of healing potential in cells derived from the peritenon (extrinsic healing) and compared these to cells from the tendon core (intrinsic healing). Both cell populations were extracted from horse superficial digital flexor tendon and characterized for tenogenic and matrix remodeling markers as well as for rates of migration and replication. Furthermore, colony-forming unit assays, multipotency assays, and real-time quantitative polymerase chain reaction analyses of markers of osteogenic and adipogenic differentiation after culture in induction media were performed. Finally, cellular capacity for differentiation towards a myofibroblastic phenotype was assessed. Our results demonstrate that both tendon- and peritenon-derived cell populations are capable of adipogenic and osteogenic differentiation, with higher expression of progenitor cell markers in peritenon cells. Cells from the peritenon also migrated faster, replicate more quickly, and show higher differentiation potential toward a myofibroblastic phenotype when compared to cells from the tendon core. Based on these data, we suggest that cells from the peritenon have substantial potential to influence tendon-healing outcome, warranting further scrutiny of their role.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
PLOS
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Differences between the Cell Populations from the Peritenon and the Tendon Core with Regard to Their Potential Implication in Tendon Repair
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
PLoS ONE
ethz.journal.volume
9
en_US
ethz.journal.issue
3
en_US
ethz.journal.abbreviated
PLoS ONE
ethz.pages.start
92474
en_US
ethz.size
11 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
San Francisco, CA
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02518 - Institut für Biomechanik / Institute for Biomechanics::03822 - Snedeker, Jess G. / Snedeker, Jess G.
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02518 - Institut für Biomechanik / Institute for Biomechanics::03822 - Snedeker, Jess G. / Snedeker, Jess G.
ethz.date.deposited
2017-06-11T07:17:44Z
ethz.source
ECIT
ethz.identifier.importid
imp593651cd2c2fe15030
ethz.ecitpid
pub:130735
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-15T07:24:05Z
ethz.rosetta.lastUpdated
2024-02-02T05:38:17Z
ethz.rosetta.versionExported
true
ethz.COinS
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