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dc.contributor.author
Altvater, Martin
dc.contributor.author
Schütz, Sabina
dc.contributor.author
Chang, Yiming
dc.contributor.author
Panse, Vikram G.
dc.contributor.editor
Doye, Valérie
dc.date.accessioned
2021-01-19T09:09:38Z
dc.date.available
2017-06-11T08:16:04Z
dc.date.available
2021-01-19T09:09:38Z
dc.date.issued
2014
dc.identifier.isbn
978-0-12-417160-2
en_US
dc.identifier.issn
0091-679X
dc.identifier.other
10.1016/B978-0-12-417160-2.00020-5
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/83929
dc.description.abstract
Construction of the eukaryotic ribosome begins in the nucleolus and requires > 300 evolutionarily conserved nonribosomal trans-acting factors, which transiently associate with preribosomal subunits at distinct assembly stages. A subset of trans-acting and transport factors passage assembled preribosomal subunits in a functionally inactive state through the nuclear pore complexes (NPC) into the cytoplasm, where they undergo final maturation before initiating translation. Here, we summarize the repertoire of tools developed in the model organism budding yeast that are spearheading the functional analyses of trans-acting factors involved in the assembly and intracellular transport of preribosomal subunits. We elaborate on different GFP-tagged ribosomal protein reporters and a pre-rRNA reporter that reliably monitors the movement of preribosomal particles from the nucleolus to cytoplasm. We discuss the powerful yeast heterokaryon assay, which can be employed to uncover shuttling trans-acting factors that need to accompany preribosomal subunits to the cytoplasm to be released prior to initiating translation. Moreover, we present two biochemical approaches, namely sucrose gradient analyses and tandem affinity purification, that are rapidly facilitating the uncovering of regulatory processes that control the compositional dynamics of trans-acting factors on maturing preribosomal particles. Altogether, these approaches when combined with traditional analytical biochemistry, targeted proteomics and structural methodologies, will contribute to the dissection of the assembly and intracellular transport of preribosomal subunits, as well as other macromolecular assemblies that influence diverse biological pathways.
en_US
dc.language.iso
en
en_US
dc.publisher
Academic Press
en_US
dc.subject
Budding yeast
en_US
dc.subject
Ribosome biogenesis
en_US
dc.subject
Ribosome export
en_US
dc.subject
Nuclear export
en_US
dc.subject
Sucrose sedimentation
en_US
dc.subject
TAP purification
en_US
dc.subject
Fluorescence in situ hybridization
en_US
dc.subject
Heterokaryon assay
en_US
dc.title
Dissecting ribosome assembly and transport in budding yeast
en_US
dc.type
Book Chapter
ethz.book.title
Nuclear Pore Complexes and Nucleocytoplasmic Transport - Methods
en_US
ethz.journal.title
Methods in Cell Biology
ethz.journal.volume
122
en_US
ethz.journal.abbreviated
Methods Cell Biol
ethz.pages.start
437
en_US
ethz.pages.end
461
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
San Diego, CA
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich, direkt::00012 - Lehre und Forschung, direkt::00007 - Departemente, direkt::02030 - Departement Biologie / Department of Biology::02517 - Institut für Biochemie (IBC) / Institute of Biochemistry (IBC)::03884 - Panse, Vikram G. (SNF-Professur) (ehemalig)
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich, direkt::00012 - Lehre und Forschung, direkt::00007 - Departemente, direkt::02030 - Departement Biologie / Department of Biology::02517 - Institut für Biochemie (IBC) / Institute of Biochemistry (IBC)::03884 - Panse, Vikram G. (SNF-Professur) (ehemalig)
ethz.date.deposited
2017-06-11T08:16:46Z
ethz.source
ECIT
ethz.identifier.importid
imp593651e2a5cbf57032
ethz.ecitpid
pub:132436
ethz.eth
yes
en_US
ethz.availability
Metadata only
en_US
ethz.rosetta.installDate
2017-07-14T18:12:06Z
ethz.rosetta.lastUpdated
2021-02-15T23:21:52Z
ethz.rosetta.versionExported
true
ethz.COinS
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