Show simple item record

dc.contributor.author
Shu, Huan
dc.contributor.author
Nakamura, Miyuki
dc.contributor.author
Siretskiy, Alexey
dc.contributor.author
Borghi, Lorenzo
dc.contributor.author
Moraes, Izabel
dc.contributor.author
Wildhaber, Thomas
dc.contributor.author
Gruissem, Wilhelm
dc.contributor.author
Hennig, Lars
dc.date.accessioned
2019-04-03T09:57:16Z
dc.date.available
2017-06-11T08:56:44Z
dc.date.available
2019-04-03T09:57:16Z
dc.date.issued
2014-03
dc.identifier.issn
1474-7596
dc.identifier.issn
1465-6906
dc.identifier.issn
1474-760X
dc.identifier.issn
1465-6914
dc.identifier.other
10.1186/gb-2014-15-4-r62
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/84594
dc.identifier.doi
10.3929/ethz-b-000084594
dc.description.abstract
Background Histone variants establish structural and functional diversity of chromatin by affecting nucleosome stability and histone-protein interactions. H3.3 is an H3 histone variant that is incorporated into chromatin outside of S-phase in various eukaryotes. In animals, H3.3 is associated with active transcription and possibly maintenance of transcriptional memory. Plant H3 variants, which evolved independently of their animal counterparts, are much less well understood. Results We profile the H3.3 distribution in Arabidopsis at mono-nucleosomal resolution using native chromatin immunoprecipitation. This results in the precise mapping of H3.3-containing nucleosomes, which are not only enriched in gene bodies as previously reported, but also at a subset of promoter regions and downstream of the 3′ ends of active genes. While H3.3 presence within transcribed regions is strongly associated with transcriptional activity, H3.3 at promoters is often independent of transcription. In particular, promoters with GA motifs carry H3.3 regardless of the gene expression levels. H3.3 on promoters of inactive genes is associated with H3K27me3 at gene bodies. In addition, H3.3-enriched plant promoters often contain RNA Pol II considerably upstream of the transcriptional start site. H3.3 and RNA Pol II are found on active as well as on inactive promoters and are enriched at strongly regulated genes. Conclusions In animals and plants, H3.3 organizes chromatin in transcribed regions and in promoters. The results suggest a function of H3.3 in transcriptional regulation and support a model that a single ancestral H3 evolved into H3 variants with similar sub-functionalization patterns in plants and animals.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
BioMed Central
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/2.0/
dc.subject
Gene body
en_US
dc.subject
Histone variant
en_US
dc.subject
Pericentric heterochromatin
en_US
dc.subject
Inactive gene
en_US
dc.subject
High transcript abundance
en_US
dc.title
Arabidopsis replacement histone variant H3.3 occupies promoters of regulated genes
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 2.0 Generic
dc.date.published
2014-03-21
ethz.journal.title
Genome Biology
ethz.journal.volume
15
en_US
ethz.journal.issue
4
en_US
ethz.journal.abbreviated
Genome biol.
ethz.pages.start
R62
en_US
ethz.size
14 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02541 - Institut für Molekulare Pflanzenbiologie / Institute of Molecular Plant Biology::03554 - Gruissem, Wilhelm / Gruissem, Wilhelm
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02541 - Institut für Molekulare Pflanzenbiologie / Institute of Molecular Plant Biology::03554 - Gruissem, Wilhelm / Gruissem, Wilhelm
ethz.date.deposited
2017-06-11T08:58:36Z
ethz.source
ECIT
ethz.identifier.importid
imp593651efa81fe17025
ethz.ecitpid
pub:133395
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-19T12:59:50Z
ethz.rosetta.lastUpdated
2019-04-03T09:57:29Z
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Arabidopsis%20replacement%20histone%20variant%20H3.3%20occupies%20promoters%20of%20regulated%20genes&rft.jtitle=Genome%20Biology&rft.date=2014-03&rft.volume=15&rft.issue=4&rft.spage=R62&rft.issn=1474-7596&1465-6906&1474-760X&1465-6914&rft.au=Shu,%20Huan&Nakamura,%20Miyuki&Siretskiy,%20Alexey&Borghi,%20Lorenzo&Moraes,%20Izabel&rft.genre=article&
 Search via SFX

Files in this item

Thumbnail

Publication type

Show simple item record