Show simple item record

dc.contributor.author
Allain, Frédéric H.-T.
dc.contributor.author
Duss, Olivier
dc.contributor.author
Michel, Erich
dc.contributor.author
Diarra Dit Konté, Nana
dc.contributor.author
Schubert, Mario
dc.date.accessioned
2019-04-04T10:29:33Z
dc.date.available
2017-06-11T09:09:22Z
dc.date.available
2019-04-04T10:29:33Z
dc.date.issued
2014-04
dc.identifier.other
10.1093/nar/gku141
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/84808
dc.identifier.doi
10.3929/ethz-b-000084808
dc.description.abstract
The carbon storage regulator/regulator of secondary metabolism (Csr/Rsm) type of small non-coding RNAs (sRNAs) is widespread throughout bacteria and acts by sequestering the global translation repressor protein CsrA/RsmE from the ribosome binding site of a subset of mRNAs. Although we have previously described the molecular basis of a high affinity RNA target bound to RsmE, it remains unknown how other lower affinity targets are recognized by the same protein. Here, we have determined the nuclear magnetic resonance solution structures of five separate GGA binding motifs of the sRNA RsmZ of Pseudomonas fluorescens in complex with RsmE. The structures explain how the variation of sequence and structural context of the GGA binding motifs modulate the binding affinity for RsmE by five orders of magnitude (∼10 nM to ∼3 mM, Kd). Furthermore, we see that conformational adaptation of protein side-chains and RNA enable recognition of different RNA sequences by the same protein contributing to binding affinity without conferring specificity. Overall, our findings illustrate how the variability in the Csr/Rsm protein–RNA recognition allows a fine-tuning of the competition between mRNAs and sRNAs for the CsrA/RsmE protein.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Oxford University Press
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/3.0/
dc.title
Molecular basis for the wide range of affinity found in Csr/Rsm protein-RNA recognition
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 3.0 Unported
dc.date.published
2014-02-21
ethz.journal.title
Nucleic acids research
ethz.journal.volume
42
en_US
ethz.journal.issue
8
en_US
ethz.pages.start
5332
en_US
ethz.pages.end
5346
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.grant
NMR structure determination of protein-RNA complexes involved in pre-mRNA editing and translation regulation
en_US
ethz.grant
NMR structure determination of protein-RNA complexes involved in pre-mRNA splicing and translation regulation
en_US
ethz.identifier.wos
ethz.identifier.nebis
000038633
ethz.publication.place
Oxford
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02517 - Institut für Biochemie / Institute of Biochemistry (IBC)::03591 - Allain, Frédéric / Allain, Frédéric
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02517 - Institut für Biochemie / Institute of Biochemistry (IBC)::03591 - Allain, Frédéric / Allain, Frédéric
ethz.grant.agreementno
133134
ethz.grant.agreementno
149921
ethz.grant.fundername
SNF
ethz.grant.fundername
SNF
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.program
Bonus of Excellence
ethz.grant.program
Projektförderung in Biologie und Medizin (Abteilung III)
ethz.date.deposited
2017-06-11T09:10:26Z
ethz.source
ECIT
ethz.identifier.importid
imp593651f3e4def32875
ethz.ecitpid
pub:133759
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-14T14:57:18Z
ethz.rosetta.lastUpdated
2020-02-15T18:16:37Z
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Molecular%20basis%20for%20the%20wide%20range%20of%20affinity%20found%20in%20Csr/Rsm%20protein-RNA%20recognition&rft.jtitle=Nucleic%20acids%20research&rft.date=2014-04&rft.volume=42&rft.issue=8&rft.spage=5332&rft.epage=5346&rft.au=Allain,%20Fr%C3%A9d%C3%A9ric%20H.-T.&Duss,%20Olivier&Michel,%20Erich&Diarra%20Dit%20Kont%C3%A9,%20Nana&Schubert,%20Mario&rft.genre=article&
 Search via SFX

Files in this item

Thumbnail

Publication type

Show simple item record