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dc.contributor.author
Torres, Maria J.
dc.contributor.author
Argandona, Montserrat
dc.contributor.author
Vargas, Carmen
dc.contributor.author
Bedmar, Eulogio J.
dc.contributor.author
Fischer, Hans-Martin
dc.contributor.author
Mesa, Socorro
dc.contributor.author
Delgado, María J.
dc.date.accessioned
2018-08-23T13:19:41Z
dc.date.available
2017-06-11T10:19:05Z
dc.date.available
2018-08-23T13:19:41Z
dc.date.issued
2014-06-20
dc.identifier.issn
1932-6203
dc.identifier.other
10.1371/journal.pone.0099011
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/86080
dc.identifier.doi
10.3929/ethz-b-000086080
dc.description.abstract
Bradyrhizobium japonicum RegSR regulatory proteins belong to the family of two-component regulatory systems, and orthologs are present in many Proteobacteria where they globally control gene expression mostly in a redox-responsive manner. In this work, we have performed a transcriptional profiling of wild-type and regR mutant cells grown under anoxic denitrifying conditions. The comparative analyses of wild-type and regR strains revealed that almost 620 genes induced in the wild type under denitrifying conditions were regulated (directly or indirectly) by RegR, pointing out the important role of this protein as a global regulator of denitrification. Genes controlled by RegR included nor and nos structural genes encoding nitric oxide and nitrous oxide reductase, respectively, genes encoding electron transport proteins such as cycA (blr7544) or cy2 (bll2388), and genes involved in nitric oxide detoxification (blr2806-09) and copper homeostasis (copCAB), as well as two regulatory genes (bll3466, bll4130). Purified RegR interacted with the promoters of norC (blr3214), nosR (blr0314), a fixK-like gene (bll3466), and bll4130, which encodes a LysR-type regulator. By using fluorescently labeled oligonucleotide extension (FLOE), we were able to identify two transcriptional start sites located at about 35 (P1) and 22 (P2) bp upstream of the putative translational start codon of norC. P1 matched with the previously mapped 5′end of norC mRNA which we demonstrate in this work to be under FixK2 control. P2 is a start site modulated by RegR and specific for anoxic conditions. Moreover, qRT-PCR experiments, expression studies with a norC-lacZ fusion, and heme c-staining analyses revealed that anoxia and nitrate are required for RegR-dependent induction of nor genes, and that this control is independent of the sensor protein RegS.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Public Library of Science
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
The Global Response Regulator RegR Controls Expression of Denitrification Genes in Bradyrhizobium japonicum
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
PLoS ONE
ethz.journal.volume
9
en_US
ethz.journal.issue
6
en_US
ethz.journal.abbreviated
PLoS ONE
ethz.pages.start
e99011
en_US
ethz.size
11 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.identifier.nebis
006206116
ethz.publication.place
S.l.
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02520 - Institut für Mikrobiologie / Institute of Microbiology::08818 - Fischer, Hans-Martin (Tit.-Prof.)
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02520 - Institut für Mikrobiologie / Institute of Microbiology::08818 - Fischer, Hans-Martin (Tit.-Prof.)
ethz.date.deposited
2017-06-11T10:19:32Z
ethz.source
ECIT
ethz.identifier.importid
imp5936520d325ad45495
ethz.ecitpid
pub:135545
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-15T02:55:10Z
ethz.rosetta.lastUpdated
2018-08-02T14:32:00Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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