Show simple item record

dc.contributor.author
Lavinder, Jason J.
dc.contributor.author
Hoi, Kam Hon
dc.contributor.author
Reddy, Sai T.
dc.contributor.author
Wine, Yariv
dc.contributor.author
Georgiu, George
dc.date.accessioned
2018-10-25T08:18:48Z
dc.date.available
2017-06-11T10:38:23Z
dc.date.available
2018-10-25T08:18:48Z
dc.date.issued
2014-06-30
dc.identifier.issn
1932-6203
dc.identifier.other
10.1371/journal.pone.0101322
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/86401
dc.identifier.doi
10.3929/ethz-b-000086401
dc.description.abstract
Rabbits have been used extensively as a model system for the elucidation of the mechanism of immunoglobulin diversification and for the production of antibodies. We employed Next Generation Sequencing to analyze Ig germline V and J gene usage, CDR3 length and amino acid composition, and gene conversion frequencies within the functional (transcribed) IgG repertoire of the New Zealand white rabbit (Oryctolagus cuniculus). Several previously unannotated rabbit heavy chain variable (VH) and light chain variable (VL) germline elements were deduced bioinformatically using multidimensional scaling and k-means clustering methods. We estimated the gene conversion frequency in the rabbit at 23% of IgG sequences with a mean gene conversion tract length of 59±36 bp. Sequencing and gene conversion analysis of the chicken, human, and mouse repertoires revealed that gene conversion occurs much more extensively in the chicken (frequency 70%, tract length 79±57 bp), was observed to a small, yet statistically significant extent in humans, but was virtually absent in mice.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Public Library of Science
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Systematic Characterization and Comparative Analysis of the Rabbit Immunoglobulin Repertoire
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
PLoS ONE
ethz.journal.volume
9
en_US
ethz.journal.issue
6
en_US
ethz.journal.abbreviated
PLoS ONE
ethz.pages.start
e101322
en_US
ethz.size
11 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.scopus
ethz.identifier.nebis
006206116
ethz.publication.place
S.l.
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::03952 - Reddy, Sai / Reddy, Sai
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::03952 - Reddy, Sai / Reddy, Sai
ethz.date.deposited
2017-06-11T10:42:28Z
ethz.source
ECIT
ethz.identifier.importid
imp5936521372d7889537
ethz.ecitpid
pub:136017
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-20T17:25:12Z
ethz.rosetta.lastUpdated
2018-09-01T04:06:58Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Systematic%20Characterization%20and%20Comparative%20Analysis%20of%20the%20Rabbit%20Immunoglobulin%20Repertoire&rft.jtitle=PLoS%20ONE&rft.date=2014-06-30&rft.volume=9&rft.issue=6&rft.spage=e101322&rft.issn=1932-6203&rft.au=Lavinder,%20Jason%20J.&Hoi,%20Kam%20Hon&Reddy,%20Sai%20T.&Wine,%20Yariv&Georgiu,%20George&rft.genre=article&
 Search via SFX

Files in this item

Thumbnail

Publication type

Show simple item record