
Open access
Autor(in)
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Datum
2014-07-08Typ
- Journal Article
ETH Bibliographie
yes
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Abstract
RNA silencing is an evolutionarily conserved mechanism triggered by double-stranded RNA that is processed into 21- to 24-nt small interfering (si)RNA or micro (mi)RNA by RNaseIII-like enzymes called Dicers. Gene regulations by RNA silencing have fundamental implications in a large number of biological processes that include antiviral defense, maintenance of genome integrity and the orchestration of cell fates. Although most generic or core components of the various plant small RNA pathways have been likely identified over the past 15 years, factors involved in RNAi regulation through post-translational modifications are just starting to emerge, mostly through forward genetic studies. A genetic screen designed to identify factors required for RNAi in Arabidopsis identified the serine/threonine protein kinase, TOUSLED (TSL). Mutations in TSL affect exogenous and virus-derived siRNA activity in a manner dependent upon its kinase activity. By contrast, despite their pleiotropic developmental phenotype, tsl mutants show no defect in biogenesis or activity of miRNA or endogenous trans-acting siRNA. These data suggest a possible role for TSL phosphorylation in the specific regulation of exogenous and antiviral RNA silencing in Arabidopsis and identify TSL as an intrinsic regulator of RNA interference. Mehr anzeigen
Persistenter Link
https://doi.org/10.3929/ethz-b-000088140Publikationsstatus
publishedExterne Links
Zeitschrift / Serie
Nucleic Acids ResearchBand
Seiten / Artikelnummer
Verlag
Oxford University PressOrganisationseinheit
03876 - Voinnet, Olivier / Voinnet, Olivier
Anmerkungen
It was possible to publish this article open access thanks to a Swiss National Licence with the publisherETH Bibliographie
yes
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