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dc.contributor.author
Ausländer, David
dc.contributor.author
Eggerschwiler, Benjamin
dc.contributor.author
Kemmer, Christian
dc.contributor.author
Geering, Barbara
dc.contributor.author
Ausländer, Simon
dc.contributor.author
Fussenegger, Martin
dc.date.accessioned
2018-09-11T11:40:15Z
dc.date.available
2017-06-11T12:17:17Z
dc.date.available
2018-09-11T11:40:15Z
dc.date.issued
2014
dc.identifier.other
10.1038/ncomms5408
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/88917
dc.identifier.doi
10.3929/ethz-b-000088917
dc.description.abstract
Allergic disorders are markedly increasing in industrialized countries. The identification of compounds that trigger the immunoglobulin E-dependent allergic reaction remains the key to limit patients’ exposure to critical allergens and improve their quality of life. Here we use synthetic biology principles to design a mammalian cell-based allergy profiler that scores the allergen-triggered release of histamine from whole-blood-derived human basophils. A synthetic signalling cascade engineered within the allergy profiler rewires histamine input to the production of reporter protein, thereby integrating histamine levels in whole-blood samples with remarkable sensitivity and a wide dynamic range, allowing for rapid results or long-term storage of output, respectively. This approach provides non-intrusive allergy profiles for the personalized medicine era.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature Publishing Group
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc-sa/4.0/
dc.title
A designer cell-based histamine-specific human allergy profiler
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International
dc.date.published
2014-08-05
ethz.journal.title
Nature Communications
ethz.journal.volume
5
en_US
ethz.pages.start
4408
en_US
ethz.size
8 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.identifier.nebis
007044158
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich, direkt::00012 - Lehre und Forschung, direkt::00007 - Departemente, direkt::02060 - Departement Biosysteme / Department of Biosystems Science and Engineering::03694 - Fussenegger, Martin
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich, direkt::00012 - Lehre und Forschung, direkt::00007 - Departemente, direkt::02060 - Departement Biosysteme / Department of Biosystems Science and Engineering::03694 - Fussenegger, Martin
ethz.date.deposited
2017-06-11T12:17:41Z
ethz.source
ECIT
ethz.identifier.importid
imp59365244c6bfd47019
ethz.ecitpid
pub:139970
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-26T04:01:36Z
ethz.rosetta.lastUpdated
2018-09-11T11:40:24Z
ethz.rosetta.versionExported
true
ethz.COinS
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