Show simple item record

dc.contributor.author
Surmann, Kristin
dc.contributor.author
Michalik, Stephan
dc.contributor.author
Hildebrandt, Petra
dc.contributor.author
Gierok, Philipp
dc.contributor.author
Depke, Maren
dc.contributor.author
Brinkmann, Lars
dc.contributor.author
Bernhardt, Jörg
dc.contributor.author
Salazar, Manuela G.
dc.contributor.author
Sun, Zhi
dc.contributor.author
Shteynberg, David
dc.contributor.author
Kusebauch, Ulrike
dc.contributor.author
Moritz, Robert L.
dc.contributor.author
Wollscheid, Bernd
dc.contributor.author
Lalk, Michael
dc.contributor.author
Völker, Uwe
dc.contributor.author
Schmidt, Frank
dc.date.accessioned
2018-11-07T12:36:10Z
dc.date.available
2017-06-11T12:17:18Z
dc.date.available
2018-11-07T12:36:10Z
dc.date.issued
2014-08-01
dc.identifier.issn
1664-302X
dc.identifier.other
10.3389/fmicb.2014.00392
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/88918
dc.identifier.doi
10.3929/ethz-b-000088918
dc.description.abstract
Staphylococcus aureus is a human pathogen that can cause a wide range of diseases. Although formerly regarded as extracellular pathogen, it has been shown that S. aureus can also be internalized by host cells and persist within these cells. In the present study, we comparatively analyzed survival and physiological adaptation of S. aureus HG001 after internalization by two human lung epithelial cell lines (S9 and A549), and human embryonic kidney cells (HEK 293). Combining enrichment of bacteria from host-pathogen assays by cell sorting and quantitation of the pathogen's proteome by mass spectrometry we characterized S. aureus adaptation during the initial phase between 2.5 h and 6.5 h post-infection. Starting with about 2 × 106 bacteria, roughly 1450 S. aureus proteins, including virulence factors and metabolic enzymes were identified by spectral comparison and classical database searches. Most of the bacterial adaptation reactions, such as decreased levels of ribosomal proteins and metabolic enzymes or increased amounts of proteins involved in arginine and lysine biosynthesis, enzymes coding for terminal oxidases and stress responsive proteins or activation of the sigma factor SigB were observed after internalization into any of the three cell lines studied. However, differences were noted in central carbon metabolism including regulation of fermentation and threonine degradation. Since these differences coincided with different intracellular growth behavior, complementary profiling of the metabolome of the different non-infected host cell types was performed. This revealed similar levels of intracellular glucose but host cell specific differences in the amounts of amino acids such as glycine, threonine or glutamate. With this comparative study we provide an impression of the common and specific features of the adaptation of S. aureus HG001 to specific host cell environments as a starting point for follow-up studies with different strain isolates and regulatory mutants.
en_US
dc.language.iso
en
en_US
dc.publisher
Fontiers Research Foundation
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/3.0/
dc.subject
Staphylococcus
en_US
dc.subject
Human cell lines
en_US
dc.subject
Host-pathogen interaction
en_US
dc.subject
Proteomics
en_US
dc.subject
Label-free quantitation
en_US
dc.title
Comparative proteome analysis reveals conserved and specific adaptation patterns of Staphylococcus aureus after internalization by different types of human non-professional phagocytic host cells
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 3.0 Unported
ethz.journal.title
Frontiers in Microbiology
ethz.journal.volume
5
en_US
ethz.journal.abbreviated
Front. microbiol.
ethz.pages.start
392
en_US
ethz.size
14 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.nebis
010181524
ethz.publication.place
Lausanne
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2017-06-11T12:17:41Z
ethz.source
ECIT
ethz.identifier.importid
imp59365244cc25c38836
ethz.ecitpid
pub:139971
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-20T16:13:32Z
ethz.rosetta.lastUpdated
2019-01-03T09:19:44Z
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Comparative%20proteome%20analysis%20reveals%20conserved%20and%20specific%20adaptation%20patterns%20of%20Staphylococcus%20aureus%20after%20internalization%20by%20differen&rft.jtitle=Frontiers%20in%20Microbiology&rft.date=2014-08-01&rft.volume=5&rft.spage=392&rft.issn=1664-302X&rft.au=Surmann,%20Kristin&Michalik,%20Stephan&Hildebrandt,%20Petra&Gierok,%20Philipp&Depke,%20Maren&rft.genre=article&
 Search via SFX

Files in this item

Thumbnail

Publication type

Show simple item record