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dc.contributor.author
Fruhwürth, Stefanie
dc.contributor.author
Kovacs, Werner
dc.contributor.author
Bittman, Robert
dc.contributor.author
Messner, Simon
dc.contributor.author
Röhrl, Clemens
dc.contributor.author
Stangl, Herbert
dc.date.accessioned
2019-12-06T07:52:28Z
dc.date.available
2017-06-11T12:19:41Z
dc.date.available
2019-12-06T07:52:28Z
dc.date.issued
2014-12
dc.identifier.issn
0948-6143
dc.identifier.issn
1432-119X
dc.identifier.other
10.1007/s00418-014-1251-9
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/89021
dc.identifier.doi
10.3929/ethz-b-000089021
dc.description.abstract
The high-density lipoprotein (HDL) receptor, scavenger receptor class B, type I (SR-BI), mediates selective cholesteryl ester uptake into the liver, which finally results in cholesterol secretion into the bile. Despite several reports, the distribution of hepatic SR-BI between the sinusoidal and canalicular membranes is still under debate. We present immunohistological data using specific markers showing that the bulk of SR-BI is present in sinusoidal membranes and, to a lesser extent, in canalicular membranes in murine and human liver sections. In addition, SR-BI was detected in preparations of rat liver canalicular membranes. We also compared the in vivo findings to HepG2 cells, a widely used in vitro hepatocyte model. Interestingly, SR-BI was enriched in bile canalicular-like (BC-like) structures in polarized HepG2 cells, which were cultivated either conventionally to form a monolayer or in Matrigel to form three-dimensional structures. Fluorescently labeled HDL was transported into close proximity of BC-like structures, whereas HDL labeled with the fluorescent cholesterol analog BODIPY-cholesterol was clearly detected within these structures. Importantly, similarly to human and mouse liver, SR-BI was localized in basolateral membranes in three-dimensional liver microtissues from primary human liver cells. Our results demonstrate that SR-BI is highly enriched in sinusoidal membranes and is also found in canalicular membranes. There was no significant basolateral–apical redistribution of hepatic SR-BI in fasting and refeeding experiments in mice. Furthermore, in vitro studies in polarized HepG2 cells showed explicit differences as SR-BI was highly enriched in BC-like structures. These structures are, however, functional and accumulated HDL-derived cholesterol. Thus, biological relevant model systems should be employed when investigating SR-BI distribution in vitro.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Springer
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
SR-BI
en_US
dc.subject
Cholesterol
en_US
dc.subject
HDL
en_US
dc.subject
Canalicular membranes
en_US
dc.subject
HepG2
en_US
dc.subject
BODIPY-cholesterol
en_US
dc.title
Differential basolateral–apical distribution of scavenger receptor, class B, type I in cultured cells and the liver
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2014-07-25
ethz.journal.title
Histochemistry and Cell Biology
ethz.journal.volume
142
en_US
ethz.journal.issue
6
en_US
ethz.journal.abbreviated
Histochem Cell Biol
ethz.pages.start
645
en_US
ethz.pages.end
655
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.scopus
ethz.identifier.nebis
001526637
ethz.publication.place
Berlin
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2017-06-11T12:20:00Z
ethz.source
ECIT
ethz.identifier.importid
imp59365246e6be295041
ethz.ecitpid
pub:140084
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-13T00:14:32Z
ethz.rosetta.lastUpdated
2019-12-06T07:52:42Z
ethz.rosetta.versionExported
true
ethz.COinS
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