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dc.contributor.author
Keel, Christoph
dc.contributor.author
Schnider, Ursula
dc.contributor.author
Maurhofer Bringolf, Monika
dc.contributor.author
Voisard, Christophe
dc.contributor.author
Laville, Jacques
dc.contributor.author
Burger, Ulrich
dc.contributor.author
Wirthner, Philippe
dc.contributor.author
Haas, Dieter
dc.contributor.author
Défago, Geneviève
dc.date.accessioned
2020-09-25T13:43:19Z
dc.date.available
2017-06-11T12:24:06Z
dc.date.available
2020-09-25T13:43:19Z
dc.date.issued
1992
dc.identifier.issn
0894-0282
dc.identifier.other
10.1094/MPMI-5-004
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/89154
dc.description.abstract
Pseudomonas fluorescens strain CHA0 suppresses Thielaviopsis basicola-induced black root rot of tobacco and Gaeumannomyces graminis var. tritici-induced take-all of wheat. Strain CHA0 produces 2,4-diacetylphloroglucinol, a metabolite with antifungal, antibacterial, and phytotoxic activity. The role of this compound in disease suppression was tested under gnotobiotic conditions. A P. fluorescens mutant, obtained by Tn5 insertion, did not produce 2,4-diacetylphloroglucinol, showed diminished inhibition of T. basicola and of G. g. var. tritici in vitro, and had a reduced suppressive effect on tobacco black root rot and on take-all of wheat, compared with wild-type CHA0. Complementation of the mutant with an 11-kb DNA fragment from a genomic library of wild-type CHA0 largely restored production of the metabolite, inhibition of the fungal pathogens in vitro and disease suppression. The Tn5 insertion was physically mapped using a 5.8-kb complementing fragment as a probe. 2,4-Diacetylphloroglucinol was shown to be produced in the rhizosphere of wheat by strain CHA0 and by the complemented mutant, but not by the mutant defective in 2,4-diacetylphloroglucinol synthesis. These results support the importance of 2,4-diacetylphloroglucinol production by strain CHA0 in the suppression of soilborne plant pathogens in the rhizosphere.
en_US
dc.language.iso
en
en_US
dc.publisher
American Phytopathological Society
en_US
dc.subject
Antibiotics
en_US
dc.subject
Biological control
en_US
dc.subject
Tn5 mutagenesis
en_US
dc.title
Suppression of root diseases by Pseudomonas fluorescens CHA0 - importance of the bacterial seconday metabolite 2,4-diacetylphloroglucinol
en_US
dc.type
Journal Article
ethz.journal.title
Molecular Plant-Microbe Interactions
ethz.journal.volume
5
en_US
ethz.journal.issue
1
en_US
ethz.journal.abbreviated
Mol. Plant-Microb. Interact.
ethz.pages.start
4
en_US
ethz.pages.end
13
en_US
ethz.identifier.nebis
000041070
ethz.publication.place
St. Paul
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02720 - Institut für Integrative Biologie / Institute of Integrative Biology::03516 - McDonald, Bruce / McDonald, Bruce
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02720 - Institut für Integrative Biologie / Institute of Integrative Biology::03516 - McDonald, Bruce / McDonald, Bruce
ethz.date.deposited
2017-06-11T12:24:58Z
ethz.source
ECIT
ethz.identifier.importid
imp59365249a8b1597963
ethz.ecitpid
pub:140249
ethz.eth
yes
en_US
ethz.availability
Metadata only
en_US
ethz.rosetta.installDate
2017-07-14T22:58:05Z
ethz.rosetta.lastUpdated
2024-02-02T12:10:22Z
ethz.rosetta.versionExported
true
ethz.COinS
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