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dc.contributor.author
Niopek, Dominik
dc.contributor.author
Benzinger, Dirk
dc.contributor.author
Roensch, Julia
dc.contributor.author
Draebing, Thomas
dc.contributor.author
Wehler, Pierre
dc.contributor.author
Eils, Roland
dc.contributor.author
Di Ventura, Barbara
dc.date.accessioned
2018-09-07T09:35:23Z
dc.date.available
2017-06-11T12:29:13Z
dc.date.available
2018-09-07T09:35:23Z
dc.date.issued
2014
dc.identifier.issn
2041-1723
dc.identifier.other
10.1038/ncomms5404
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/89405
dc.identifier.doi
10.3929/ethz-b-000089405
dc.description.abstract
The function of many eukaryotic proteins is regulated by highly dynamic changes in their nucleocytoplasmic distribution. The ability to precisely and reversibly control nuclear translocation would, therefore, allow dissecting and engineering cellular networks. Here we develop a genetically encoded, light-inducible nuclear localization signal (LINuS) based on the LOV2 domain of Avena sativa phototropin 1. LINuS is a small, versatile tag, customizable for different proteins and cell types. LINuS-mediated nuclear import is fast and reversible, and can be tuned at different levels, for instance, by introducing mutations that alter AsLOV2 domain photo-caging properties or by selecting nuclear localization signals (NLSs) of various strengths. We demonstrate the utility of LINuS in mammalian cells by controlling gene expression and entry into mitosis with blue light.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature Publishing Group
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Engineering light-inducible nuclear localization signals for precise spatiotemporal control of protein dynamics in living cells
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2014-07-14
ethz.journal.title
Nature Communications
ethz.journal.volume
5
en_US
ethz.journal.abbreviated
Nat Commun
ethz.pages.start
4404
en_US
ethz.size
11 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.scopus
ethz.identifier.nebis
007044158
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::03921 - Khammash, Mustafa / Khammash, Mustafa
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::03921 - Khammash, Mustafa / Khammash, Mustafa
ethz.date.deposited
2017-06-11T12:29:37Z
ethz.source
ECIT
ethz.identifier.importid
imp5936524f201bd70193
ethz.ecitpid
pub:140663
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-12T22:06:48Z
ethz.rosetta.lastUpdated
2019-02-03T05:26:46Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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