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dc.contributor.author
Smith, Jenna E.
dc.contributor.author
Alvarez-Dominguez, Juan R.
dc.contributor.author
Kline, Nicolas
dc.contributor.author
Huynh, Nathan J.
dc.contributor.author
Geisler, Sarah
dc.contributor.author
Hu, Wenqian
dc.contributor.author
Coller, Jeff
dc.contributor.author
Baker, Kristian E.
dc.date.accessioned
2018-09-17T09:17:30Z
dc.date.available
2017-06-11T12:29:15Z
dc.date.available
2018-09-17T09:17:30Z
dc.date.issued
2014-06
dc.identifier.other
10.1016/j.celrep.2014.05.023
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/89409
dc.identifier.doi
10.3929/ethz-b-000089409
dc.description.abstract
High-throughput gene expression analysis has revealed a plethora of previously undetected transcripts in eukaryotic cells. In this study, we investigate >1,100 unannotated transcripts in yeast predicted to lack protein-coding capacity. We show that a majority of these RNAs are enriched on polyribosomes akin to mRNAs. Ribosome profiling demonstrates that many bind translocating ribosomes within predicted open reading frames 10–96 codons in size. We validate expression of peptides encoded within a subset of these RNAs and provide evidence for conservation among yeast species. Consistent with their translation, many of these transcripts are targeted for degradation by the translation-dependent nonsense-mediated RNA decay (NMD) pathway. We identify lncRNAs that are also sensitive to NMD, indicating that translation of noncoding transcripts also occurs in mammals. These data demonstrate transcripts considered to lack coding potential are bona fide protein coding and expand the proteome of yeast and possibly other eukaryotes.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Elsevier
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc-nd/3.0/
dc.title
Translation of Small Open Reading Frames within Unannotated RNA Transcripts in Saccharomyces cerevisiae
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported
ethz.journal.title
Cell Reports
ethz.journal.volume
7
en_US
ethz.journal.issue
6
en_US
ethz.pages.start
1858
en_US
ethz.pages.end
1866
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.scopus
ethz.identifier.nebis
007612702
ethz.publication.place
New York, NY
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2017-06-11T12:29:37Z
ethz.source
ECIT
ethz.identifier.importid
imp5936524f3575522184
ethz.ecitpid
pub:140667
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-13T12:46:39Z
ethz.rosetta.lastUpdated
2018-09-17T09:17:36Z
ethz.rosetta.versionExported
true
ethz.COinS
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