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dc.contributor.author
Kaczmarczyk, Andreas
dc.contributor.author
Vorholt, Julia A.
dc.contributor.author
Francez-Charlot, Anne
dc.date.accessioned
2018-09-12T14:41:45Z
dc.date.available
2017-06-11T12:57:27Z
dc.date.available
2018-09-12T14:41:45Z
dc.date.issued
2014-09-29
dc.identifier.issn
2045-2322
dc.identifier.other
10.1038/srep06453
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/90618
dc.identifier.doi
10.3929/ethz-b-000090618
dc.description.abstract
Regulated promoters are an important basic genetic tool allowing, for example, gene-dosage and gene depletion studies. We have previously described a cumate-inducible promoter (PQ5) that is functional in diverse Alphaproteobacteria. This promoter has been engineered by combining a synthetic minimal promoter, Psyn2, and operator sites and the repressor of the Pseudomonas putida F1 cym/cmt system. In the present study, we engineered a vanillate-regulated promoter using Psyn2 and the regulatory elements of the Caulobacter crescentus vanR-vanAB system. We show that the resulting promoter, which we called PV10, responds rapidly to the inducer vanillate with an induction ratio of about two orders of magnitude in Sphingomonas melonis Fr1. In contrast to the switch-like behavior of PQ5, PV10 shows a linear dose-response curve at intermediate vanillate concentrations, allowing graded gene expression. PV10 is functionally compatible with and independent of PQ5 and cumate, and vice versa, suggesting that both systems can be used simultaneously.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature Publishing Group
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject
Bacterial techniques and applications
en_US
dc.subject
Bacterial genes
en_US
dc.title
Synthetic vanillate-regulated promoter for graded gene expression in Sphingomonas
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
ethz.journal.title
Scientific Reports
ethz.journal.volume
4
en_US
ethz.journal.abbreviated
Sci Rep
ethz.pages.start
6453
en_US
ethz.size
4 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.grant
Investigation of the alphaproteobacterial general stress response in the context of the phyllosphere environment
en_US
ethz.identifier.wos
ethz.identifier.nebis
006751867
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02520 - Institut für Mikrobiologie / Institute of Microbiology::03740 - Vorholt, Julia / Vorholt, Julia
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02520 - Institut für Mikrobiologie / Institute of Microbiology::03740 - Vorholt, Julia / Vorholt, Julia
ethz.grant.agreementno
152835
ethz.grant.fundername
SNF
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.program
Bonus of Excellence
ethz.date.deposited
2017-06-11T12:57:30Z
ethz.source
ECIT
ethz.identifier.importid
imp59365265052b327525
ethz.ecitpid
pub:142634
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-12T18:42:31Z
ethz.rosetta.lastUpdated
2021-02-15T01:44:02Z
ethz.rosetta.versionExported
true
ethz.COinS
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