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dc.contributor.author
Riddell, Carolyn E.
dc.contributor.author
Garces, Juan D.L.
dc.contributor.author
Adams, Sally
dc.contributor.author
Barribeau, Seth M.
dc.contributor.author
Twell, David
dc.contributor.author
Mallon, Eamonn B.
dc.date.accessioned
2018-10-01T13:13:21Z
dc.date.available
2017-06-11T14:49:05Z
dc.date.available
2018-10-01T13:13:21Z
dc.date.issued
2014-11
dc.identifier.other
10.1186/1471-2164-15-1031
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/94793
dc.identifier.doi
10.3929/ethz-b-000094793
dc.description.abstract
Background Ecological studies routinely show genotype-genotype interactions between insects and their parasites. The mechanisms behind these interactions are not clearly understood. Using the bumblebee Bombus terrestris/trypanosome Crithidia bombi model system (two bumblebee colonies by two Crithidia strains), we have carried out a transcriptome-wide analysis of gene expression and alternative splicing in bees during C. bombi infection. We have performed four analyses, 1) comparing gene expression in infected and non-infected bees 24 hours after infection by Crithidia bombi, 2) comparing expression at 24 and 48 hours after C. bombi infection, 3) determining the differential gene expression associated with the bumblebee-Crithidia genotype-genotype interaction at 24 hours after infection and 4) determining the alternative splicing associated with the bumblebee-Crithidia genotype-genotype interaction at 24 hours post infection. Results We found a large number of genes differentially regulated related to numerous canonical immune pathways. These genes include receptors, signaling pathways and effectors. We discovered a possible interaction between the peritrophic membrane and the insect immune system in defense against Crithidia. Most interestingly, we found differential expression and alternative splicing of immunoglobulin related genes (Dscam and Twitchin) are associated with the genotype-genotype interactions of the given bumblebee colony and Crithidia strain. Conclusions In this paper we have shown that the expression and alternative splicing of immune genes is associated with specific interactions between different host and parasite genotypes in this bumblebee/trypanosome model.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
BioMed Central
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Genotype-genotype
en_US
dc.subject
Peritrophic membrane
en_US
dc.subject
Social insects
en_US
dc.title
Differential gene expression and alternative splicing in insect immune specificity
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
BMC Genomics
ethz.journal.volume
15
en_US
ethz.pages.start
1031
en_US
ethz.size
15 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.nebis
004256340
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2017-06-11T14:49:34Z
ethz.source
ECIT
ethz.identifier.importid
imp593652b22cc2587293
ethz.ecitpid
pub:148891
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-12T20:22:11Z
ethz.rosetta.lastUpdated
2018-10-01T13:13:28Z
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true
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true
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